Sensitive and Convenient Detection of microRNAs Based on Cascade Amplification by Catalytic DNAzymes

被引：85

作者：

Tian, Tian ^{[1
]}

Xiao, Heng ^{[1
]}

Zhang, Zhengan ^{[1
]}

Long, Yuelin ^{[1
]}

Peng, Shuang ^{[1
]}

Wang, Shaoru ^{[1
]}

Zhou, Xiang ^{[1
,2
]}

Liu, Songmei ^{[3
]}

Zhou, Xin ^{[3
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Key Lab Biomed Polymers, Minist Educ, Wuhan 430072, Hubei, Peoples R China

[2] Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing, Peoples R China

[3] Wuhan Univ, Zhongnan Hosptial, Wuhan 430072, Hubei, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2013年 / 19卷 / 01期

关键词：

DNAzyme; DSN enzyme; nucleic acids; RNA; peroxidases; FLUORESCENCE DETECTION; MICROARRAY DETECTION; MOLECULAR BEACONS; SMALL RNAS; LABEL-FREE; IN-VIVO; RT-PCR; ASSAY; DNA; QUANTIFICATION;

D O I：

10.1002/chem.201203344

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

On target: We have developed two cascade amplification strategies that combine duplex specific nuclease (DSN) amplicon with either G-quadruplex-based DNA peroxidase or 8-17 DNAzyme amplicon for miRNA detection. In this way, sensitive and convenient detection of miRNAs was achieved. In the DNA peroxidase-based system, a visual color change could be observed in the presence of target miRNAs (see scheme).

引用

页码：92 / 95

页数：4

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