Quantum Dots-Based Immunofluorescent Imaging of Stromal Fibroblasts Caveolin-1 and Light Chain 3B Expression and Identification of Their Clinical Significance in Human Gastric Cancer

被引:29
作者
He, Yuyu [2 ]
Zhao, Xianda [2 ]
Gao, Jun [3 ]
Fan, Lifang [2 ]
Yang, Guifang [4 ]
Cho, William Chi-shing [1 ]
Chen, Honglei [2 ,5 ]
机构
[1] Queen Elizabeth Hosp, Dept Clin Oncol, Kowloon, Hong Kong, Peoples R China
[2] Wuhan Univ, Sch Basic Med Sci, Dept Pathol, Wuhan 430071, Peoples R China
[3] Wuhan Nano Tumor Diag Engn Res Ctr, Dept Mol Pathol, Wuhan 430075, Peoples R China
[4] Wuhan Univ, Zhongnan Hosp, Dept Pathol, Wuhan 430071, Peoples R China
[5] Rush Univ, Med Ctr, Dept Biochem, Chicago, IL 60612 USA
关键词
autophagy; cancer-associated fibroblast; caveolin-1; gastric cancer; light chain 3B; quantum dots; tumor microenvironment; EARLY TUMOR RECURRENCE; BREAST-CANCER; PROGNOSTIC-SIGNIFICANCE; AUTOPHAGY; CARCINOMA; GROWTH; CELLS; ABSENCE; MICROENVIRONMENT; METABOLISM;
D O I
10.3390/ijms131113764
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Caveolin-1 (Cav-1) expression deficiency and autophagy in tumor stromal fibroblasts (hereafter fibroblasts) are involved in tumor proliferation and progression, particularly in breast and prostate cancer. The aim of this study was to detect the expression of fibroblastic Cav-1 and LC3B, markers of autophagy, in gastric cancer (GC) and to analyze their clinical significances. Furthermore, because Epstein-Barr virus (EBV)-associated GC (EBVaGC) is a unique subtype of GC; we compared the differential expression of fibroblastic Cav-1 and LC3B in EBVaGC and non-EBVaGC. Quantum dots (QDs)-based immunofluorescence histochemistry was used to examine the expression of fibroblastic Cav-1 and LC3B in 118 cases of GC with adequate stroma. QDs-based double immunofluorescence labeling was performed to detect the coexpression of Cav-1 and LC3B proteins. EBV-encoded small RNA was detected by QDs-based fluorescence in situ hybridization to identify EBVaGC. Multivariate analysis indicated that low fibroblastic Cav-1 level was an independent prognosticator (p = 0.029) that predicted poorer survival of GC patients. Positive fibroblastic LC3B was correlated with lower invasion (p = 0.032) and was positively associated with Cav-1 expression (r = 0.432, p < 0.001). EBV infection did not affect fibroblastic Cav-1 and LC3B expression. In conclusion, positive fibroblastic LC3B correlates with lower invasion, and low expression of fibroblastic Cav-1 is a novel predictor of poor GC prognosis.
引用
收藏
页码:13764 / 13780
页数:17
相关论文
共 47 条
[1]   Stromal expression of actin is a marker of aggressiveness in basal cell carcinoma [J].
Adegboyega, Patrick A. ;
Rodriguez, Sarah ;
McLarty, Jerry .
HUMAN PATHOLOGY, 2010, 41 (08) :1128-1137
[2]   Epstein-Barr virus associated gastric carcinoma: Epidemiological and clinicopathological features [J].
Akiba, Suminori ;
Koriyama, Chihaya ;
Herrera-Goepfert, Roberto ;
Eizuru, Yoshito .
CANCER SCIENCE, 2008, 99 (02) :195-201
[3]   Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].
Chan, WCW ;
Nie, SM .
SCIENCE, 1998, 281 (5385) :2016-2018
[4]  
Chen C, 2012, NANOMEDICINE-UK, V7, P411, DOI [10.2217/NNM.12.9, 10.2217/nnm.12.9]
[5]   Quantum-dot-based immunofluorescent imaging of HER2 and ER provides new insights into breast cancer heterogeneity [J].
Chen, Chuang ;
Peng, Jun ;
Xia, Heshun ;
Wu, Qiongshui ;
Zeng, Libo ;
Xu, Hao ;
Tang, Hongwu ;
Zhang, Zhiling ;
Zhu, Xiaobo ;
Pang, Daiwen ;
Li, Yan .
NANOTECHNOLOGY, 2010, 21 (09)
[6]   Quantum dots-based immunofluorescence technology for the quantitative determination of HER2 expression in breast cancer [J].
Chen, Chuang ;
Peng, Jun ;
Xia, He-Shun ;
Yang, Gui-Fang ;
Wu, Qjong-Shui ;
Chen, Liang-Dong ;
Zeng, Li-Bo ;
Zhang, Zhi-Ling ;
Pang, Dai-Wen ;
Li, Yan .
BIOMATERIALS, 2009, 30 (15) :2912-2918
[7]   Comparison of quantum dots immunofluorescence histochemistry and conventional immunohistochemistry for the detection of caveolin-1 and PCNA in the lung cancer tissue microarray [J].
Chen, Honglei ;
Xue, Jingling ;
Zhang, Yuxia ;
Zhu, Xiaobo ;
Gao, Jun ;
Yu, Baoping .
JOURNAL OF MOLECULAR HISTOLOGY, 2009, 40 (04) :261-268
[8]   Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth [J].
Chiavarina, Barbara ;
Whitaker-Menezes, Diana ;
Martinez-Outschoorn, Ubaldo E. ;
Witkiewicz, Agnieszka K. ;
Birbe, Ruth C. ;
Howell, Anthony ;
Pestell, Richard G. ;
Smith, Johanna ;
Daniel, Rene ;
Sotgia, Federica ;
Lisanti, Michael P. .
CANCER BIOLOGY & THERAPY, 2011, 12 (12) :1101-1113
[9]   Autophagy and cancer [J].
Choi, Kyeong Sook .
EXPERIMENTAL AND MOLECULAR MEDICINE, 2012, 44 (02) :109-120
[10]   An absence of stromal caveolin-1 is associated with advanced prostate cancer, metastatic disease and epithelial Akt activation [J].
Di Vizio, Dolores ;
Morello, Matteo ;
Sotgia, Federica ;
Pestell, Richard G. ;
Freeman, Michael R. ;
Lisanti, Michael P. .
CELL CYCLE, 2009, 8 (15) :2420-2424