Small-RNA-Mediated Genome-wide trans-Recognition Network in Tetrahymena DNA Elimination

被引：41

作者：

Noto, Tomoko ^{[1
]}

Kataoka, Kensuke ^{[1
]}

Suhren, Jan H. ^{[1
]}

Hayashi, Azusa ^{[1
]}

Woolcock, Katrina J. ^{[1
]}

Gorovsky, Martin A. ^{[2
]}

Mochizuki, Kazufumi ^{[1
]}

机构：

[1] Austrian Acad Sci IMBA, Inst Mol Biotechnol, A-1030 Vienna, Austria

[2] Univ Rochester, Dept Biol, Rochester, NY 14627 USA

来源：

MOLECULAR CELL | 2015年 / 59卷 / 02期

基金：

奥地利科学基金会; 欧洲研究理事会;

关键词：

GERMLINE GENE-EXPRESSION; DICER-LIKE PROTEIN; REARRANGEMENT; THERMOPHILA; DELETION; DEFENSE; TRANSCRIPTION; METHYLATION; BIOGENESIS; BOUNDARIES;

D O I：

10.1016/j.molcel.2015.05.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SmallRNAs are usedto silence transposable elements (TEs) in many eukaryotes, which use diverse evolutionary solutions to identify TEs. In ciliated protozoans, small-RNA-mediated comparison of the germline and somatic genomes underlies identification of TE-related sequences, which are then eliminated fromthe soma. Here, we describe an additionalmechanism of small-RNA-mediated identification of TErelated sequences in the ciliate Tetrahymena. We show that a limited set of internal eliminated sequences (IESs) containing potentially active TEs produces a class of small RNAs that recognize not only the IESs from which they are derived, but also other IESs in trans. This trans recognition triggers the expression of yet another class of small RNAs that identify other IESs. Therefore, TE-related sequences in Tetrahymena are robustly targeted for elimination by a genome-wide trans-recognition network accompanied by a chain reaction of small RNA production.

引用

页码：229 / 242

页数：14

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