Inhibition of arachidonate lipoxygenase activities by 2-(3,4-dihydroxyphenyl)ethanol, a phenolic compound from olives

被引:104
作者
Kohyama, N
Nagata, T
Fujimoto, S
Sekiya, K
机构
[1] SHIKOKU NATL AGR EXPT STN, ZENTSUJI, KAGAWA 765, JAPAN
[2] NATL FOOD RES INST, TSUKUBA, IBARAKI 305, JAPAN
关键词
arachidonic acid; lipoxygenase; inhibitor; olive; phenolic compounds;
D O I
10.1271/bbb.61.347
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of olive fruit extract on arachidonic acid lipoxygenase activities were investigated using rat platelets and rat polymorphonuclear leukocytes (PMNL), Olive extract strongly inhibited both 12-lipoxygenase (12-LO) and 5-lipoxygenase (5-LO) activities, One of the compounds responsible for this inhibition was purified and identified as 2-(3,4-dihydroxyphenyl)ethanol (DPE), DPE inhibited platelet 12-LO activity (IC50, 4.2 mu M) and PMNL 5-LO activity (IC50, 13 mu M) but not cyclooxygenase activity in cell-free conditions, It also inhibited 12-LO activity in intact platelets (IC50, 50 mu M) and reduced leukotriene B-4 production in intact PMNL stimulated by A23187 (IC50, 26 mu M), The inhibition by DPE of both lipoxygenase activities was stronger than that by oleuropein, caffeic acid, or 7 other related phenolic compounds, especially in intact cells, These results suggest that DPE is a potent specific inhibitor of lipoxygenase activities.
引用
收藏
页码:347 / 350
页数:4
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