Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB

被引：41

作者：

Aksenova, Vasilisa ^{[1
,2
]}

Turoverova, Lidia ^{[1
]}

Khotin, Mikhail ^{[1
]}

Magnusson, Karl-Eric ^{[3
]}

Tulchinsky, Eugene ^{[4
]}

Melino, Gerry ^{[2
,5
]}

Pinaev, George P. ^{[1
]}

Barlev, Nickolai ^{[1
,2
,6
]}

Tentler, Dmitri ^{[1
,2
]}

机构：

[1] Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia

[2] St Petersburg Technol Inst, Mol Pharmacol Lab, St Petersburg, Russia

[3] Linkoping Univ, Div Med Microbiol, Dept Clin & Expt Med, SE-58185 Linkoping, Sweden

[4] Univ Leicester, Dept Canc Studies & Mol Med, Leicester, Leics, England

[5] MRC, Toxicol Unit, Leicester, Leics, England

[6] Univ Leicester, Dept Biochem, Leicester LE1 7RH, Leics, England

来源：

ONCOTARGET | 2013年 / 4卷 / 02期

关键词：

ACTN4; RelA/p65; transcription regulation; MMP; CELL-CYCLE ARREST; FACTOR-KAPPA-B; GENE-TRANSCRIPTION; CANCER; INHIBITION; APOPTOSIS; ACTIVATION; TNF; PHOSPHORYLATION; ALPHA-ACTININ-4;

D O I：

10.18632/oncotarget.901

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

ACTN4 is an actin-binding protein that participates in cytoskeleton organisation. It resides both in the cytoplasm and nucleus and physically associates with various transcription factors. Here, we describe an effect of ACTN4 expression on transcriptional activity of the RelA/p65 subunit of NF-kB. We demonstrate that ACTN4 enhances RelA/p65-dependant expression of c-fos, MMP-3 and MMP-1 genes, but it does not affect TNC, ICAM1 and FN1 expression. Importantly, actin-binding domains of ACTN4 are not critical for the nuclear translocation and co-activation of RelA/p65-dependent transcription. Collectively, our data suggest that in the nucleus, ACTN4 functions as a selective transcriptional co-activator of RelA/p65.

引用

页码：362 / 372

页数：11

共 61 条

[1] Oxidative Stress-Mediated Induction of MMP-1 and MMP-3 in Human RPE Cells [J].

Alge-Priglinger, Claudia S. ;

Kreutzer, Thomas ;

Obholzer, Katja ;

Wolf, Armin ;

Mempel, Martin ;

Kernt, Marcus ;

Kampik, Anselm ;

Priglinger, Siegfried G. .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2009, 50 (11) :5495-5503

[2] The p65/RelA subunit of NF-κB interacts with actin-containing structures [J].

Are, AF ;

Galkin, VE ;

Pospelova, TV ;

Pinaev, GP .

EXPERIMENTAL CELL RESEARCH, 2000, 256 (02) :533-544

[3] RelA/NF-κB transcription factor associates with α-actinin-4 [J].

Babakov, Vladimir N. ;

Petukhova, Olga A. ;

Turoverova, Lidia V. ;

Kropacheva, Irina V. ;

Tentler, Dmitri G. ;

Bolshakova, Anastasia V. ;

Podolskaya, Ekaterina P. ;

Magnusson, Karl-Eric ;

Pinaev, George P. .

EXPERIMENTAL CELL RESEARCH, 2008, 314 (05) :1030-1038

[4] The RelB subunit of NFκB acts as a negative regulator of circadian gene expression [J].

Bellet, Marina M. ;

Zocchi, Loredana ;

Sassone-Corsi, Paolo .

CELL CYCLE, 2012, 11 (17) :3304-3311

[5] Inhibition of transcription factor NF-κB reduces matrix metalloproteinase-1,-3 and-9 production by vascular smooth muscle cells [J].

Bond, M ;

Chase, AJ ;

Baker, AH ;

Newby, AC .

CARDIOVASCULAR RESEARCH, 2001, 50 (03) :556-565

[6] Spectrin, α-actinin, and dystrophin [J].

Broderick, MJF ;

Winder, SJ .

FIBROUS PROTEINS: COILED-COILS, COLLAGEN AND ELASTOMERS, 2005, 70 :203-+

[7] α-actinin 4 potentiates myocyte enhancer factor-2 transcription activity by antagonizing histone deacetylase 7 [J].

Chakraborty, Sharmistha ;

Reineke, Erin L. ;

Lam, Minh ;

Li, Xiaofang ;

Liu, Yu ;

Gao, Chengzhuo ;

Khurana, Simran ;

Kao, Hung-Ying .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (46) :35070-35080

[8] Inhibition of Aurora-A kinase induces cell cycle arrest in epithelial ovarian cancer stem cells by affecting NFκB pathway [J].

Chefetz, Ilana ;

Holmberg, Jennie C. ;

Alvero, Ayesha B. ;

Visintin, Irene ;

Mor, Gil .

CELL CYCLE, 2011, 10 (13) :2206-2214

[9] Gaseous Nitrogen Oxide Promotes Human Lung Cancer Cell Line A549 Migration, Invasion, and Metastasis via iNOS-Mediated MMP-2 Production [J].

Chen, Jing-Hsien ;

Lin, Hui-Hsuan ;

Chiang, Tai-An ;

Hsu, Jeng-Dong ;

Ho, Hsieh-Hsun ;

Lee, Yi-Chieh ;

Wang, Chau-Jong .

TOXICOLOGICAL SCIENCES, 2008, 106 (02) :364-375

[10]

Christerson LB, 1999, CELL MOTIL CYTOSKEL, V43, P186, DOI 10.1002/(SICI)1097-0169(1999)43:3<186::AID-CM2>3.3.CO

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