Ammosamides A and B Target Myosin

被引：86

作者：

Hughes, Chambers C. ^{[1
]}

MacMillan, John B. ^{[1
]}

Gaudencio, Susana P. ^{[1
]}

Fenical, William ^{[1
]}

La Clair, James J. ^{[2
]}

机构：

[1] Univ Calif San Diego, Scripps Inst Oceanog, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 USA

[2] Xenobe Res Inst, San Diego, CA 92116 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 04期

关键词：

actinomycetes; cell cycle inhibitors; mitosis; myosin; natural products; NATURAL-PRODUCTS; INHIBITION; CYTOSKELETON; VISUALIZATION; CELLS; TOOL;

D O I：

10.1002/anie.200804107

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The protein target of the ammosamides, cytotoxic natural products from a marine-derived actinomycete, has been elucidated. An immunoaffinity fluorescent tag was used to construct a fluorescent molecular probe (see structure). First, uptake and localization of the probe in cells was visualized with fluorescence microscopy. The probe was then used to co-immunoprecipitate proteins that bound to the ammosamide core. Myosin was thus identified as a target. (Figure Presented). © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：728 / 732

页数：5

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