This study investigated and described the nature and extent of inter-professional collaboration between the Traditional Health Practitioners (THPs) and Community Nurses (CNs) in Primary Health Care (PHC) in the Ngaka Modiri Molema (NMM) District of North West Province, South Africa. A cross-sectional survey design was used in this study. Data was collected with a self- constructed questionnaire from 264 CNs and 217 THPs in the five sub districts of NMM. Descriptive data was analyzed using Statistical Package for Social Science version 20. Majority (76%) of healthcare professionals were females. There was no significant relationship between the respondents' age, gender and educational level towards inter-professional collaboration (p>0.05). About 162 (82, 2%) of CNs and 205 (95, 8%) of THPs indicated that they respect each other as health care professionals in their own right. The high percentage of respect was an expectation of positive inter-professional collaboration. Respect is key element to success between and among the professionals. About 69 (6%) of CNs would not advise patients to consult THPs and (97, 2%) of THPs would advise patients to consult CNs. About 191(95 %) of CNs have never referred patients to THPs. About 204 (95, 3 %) of the THPs indicated that they will refer patients to the CNs without fear, guilt or insecurity. About 177 (68, 8%) of CNs and 148 (92, 1%) of THPs support working together. This study revealed that the there was no significant association between inter-professional collaboration between the THPs and CNs at PHC in Ngaka Modiri Molema District; however, there was willingness by healthcare professionals to collaborate.