Cysteine biosynthesis in higher plants: A new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph

被引：29

作者：

Howarth, JR ^{[1
]}

Roberts, MA ^{[1
]}

Wray, JL ^{[1
]}

机构：

[1] UNIV ST ANDREWS,SCH BIOL & MED SCI,RES DIV ENVIRONM & EVOLUT BIOL,PLANT SCI LAB,ST ANDREWS FK16 9TH,FIFE,SCOTLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1350卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

serine acetyltransferase; cysteine biosynthesis; nucleotide sequence; sulfate regulation;

D O I：

10.1016/S0167-4781(96)00213-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone, Sat-52, encoding a novel isoform of serine acetyltransferase (EC 2.3.1.30) was isolated by functional complementation of an Escherichia coli cysE mutant defective in serine acetyltransferase. The 1158 base pair clone contains a full-length open reading frame encoding a deduced protein of 312 amino acids with an M(r) of 32.77 kDa. Northern analysis revealed a single transcript of ca 1.19 kb that did not increase in abundance under sulfate limitation. Genomic Southern hybridization suggests the presence of a single copy of the Sat-52 gene.

引用

页码：123 / 127

页数：5

共 27 条

[1] A NEW MEMBER OF THE CYTOSOLIC O-ACETYLSERINE(THIOL)LYASE GENE FAMILY IN ARABIDOPSIS-THALIANA [J].