Identification and Molecular Tagging of Leaf Rust Resistance Gene (Lr24) in Wheat

被引:1
|
作者
Zhang Na [1 ]
Yang Wen-xiang [1 ]
Liu Da-qun [1 ]
机构
[1] Agr Univ Hebei, Dept Plant Pathol, Biol Control Ctr Plant Dis & Plant Pests Hebei Pr, Baoding 071001, Peoples R China
来源
AGRICULTURAL SCIENCES IN CHINA | 2011年 / 10卷 / 12期
基金
高等学校博士学科点专项科研基金; 中国国家自然科学基金;
关键词
wheat leaf rust; Lr24; gene; AFLP; STS; MAS; STS MARKERS; AFLP MARKERS; SCAR MARKER; CONVERSION; FERTILITY; LOCUS; VALIDATION; SELECTION; REGIONS; LR19;
D O I
10.1016/S1671-2927(11)60190-9
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection (MAS). An F-2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker. A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents, as well as the resistant bulk and the susceptible bulk. Four AFLP markers, P-AGA/M-CTT289 bp, P-AGC/M-CAC(188) (bp), P-AGC/M-CAC(162) (bp), and P-ACG/M-CGC(239) (bp), were co-segregated with Lr24. The AFLP fragment from the primer combination P-ACG/M-CGC was cloned, sequenced and converted into a STS marker named as ASTS212. Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS1302(607) developed by Gupta. 5R615, 5R616, 1R13, and 1R17 were identified and validated to contain gene Lr24. The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.
引用
收藏
页码:1898 / 1905
页数:8
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