Extraction of Astaxanthin from Shrimp Waste using Response Surface Methodology and a New Hybrid Organic-Inorganic Monolith

A 17-run Box-Behnken design (BBD) with three factors was used to improve the conditions for extracting astaxanthin from shrimp waste. The astaxanthin level was determined by high performance liquid chromatography (HPLC) using a C-18 column and a UV detector at 476nm. The mean extraction yield of astaxanthin at the improved conditions (extraction time 1.4h, extraction temperature 72.4 degrees C, and liquid-solid ratio 27.3) was 98.7 +/- 2.6 mu gg(1). A solid-phase extraction (SPE)-HPLC method was developed with a new hybrid organic-inorganic hybrid monolith for further purification of astaxanthin from shrimp waste. The SPE recoveries were ranging from 81.3 +/- 2.4% to 86.5 +/- 3.3%, and the intra-day and inter-day relative standard deviations (RSDs) of the proposed method were less than 4.3 +/- 0.5% and 4.8 +/- 0.2%, respectively. BBD increased the extraction efficiency and shortened extraction times significantly. SPE-HPLC with hybrid monolith showed good selectivity and purified astaxanthin from shrimp waste.