The pentose catabolic pathway of the rice-blast fungus Magnaporthe oryzae involves a novel pentose reductase restricted to few fungal species

被引：12

作者：

Klaubauf, Sylvia ^{[1
,3
]}

Ribot, Cecile ^{[2
,3
]}

Melayah, Delphine ^{[2
,3
]}

Lagorce, Arnaud ^{[2
,3
]}

Lebrun, Marc-Henri ^{[3
,4
]}

de Vries, Ronald P. ^{[1
,3
,5
]}

机构：

[1] CBS KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands

[2] Bayer Cropsci, Lyon, France

[3] Bayer Crop Sci, MPA, UMR 2847, CNRS, Lyon, France

[4] INRA, BIOGER, UR 1290, F-78850 Thiverval Grignon, France

[5] Microbiol & Kluyver Ctr Genom Ind Fermentat, Utrecht, Netherlands

来源：

FEBS LETTERS | 2013年 / 587卷 / 09期

关键词：

Pentose catabolism; Pentose reductase; D-Xylose; L-Arabinose; Magnaporthe oryzae; L-ARABINITOL; 4-DEHYDROGENASE; D-GALACTOSE CATABOLISM; D-XYLOSE REDUCTASE; ASPERGILLUS-NIGER; L-ARABINOSE; HYPOCREA-JECORINA; MISSING LINK; GENE; EXPRESSION; GRISEA;

D O I：

10.1016/j.febslet.2013.03.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene (MoPRD1), related to xylose reductases, was identified in Magnaporthe oryzae. Recombinant MoPRD1 displays its highest specific reductase activity toward L-arabinose and D-xylose. K-m and V-max values using L-arabinose and D-xylose are similar. MoPRD1 was highly overexpressed 2-8 h after transfer of mycelium to D-xylose or L-arabinose, compared to D-glucose. Therefore, we conclude that MoPDR1 is a novel pentose reductase, which combines the activities and expression patterns of fungal L-arabinose and D-xylose reductases. Phylogenetic analysis shows that PRD1 defines a novel family of pentose reductases related to fungal D-xylose reductases, but distinct from fungal L-arabinose reductases. The presence of PRD1, L-arabinose and D-xylose reductases encoding genes in a given species is variable and likely related to their life style. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1346 / 1352

页数：7

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