Development of a Method for the Analysis of Multiclass Antibiotic Residues in Milk Using QuEChERS and Liquid Chromatography-Tandem Mass Spectrometry

被引：28

作者：

Wang, Yuan-long ^{[1
,2
,3
]}

Liu, Zhen-min ^{[1
,2
,3
]}

Ren, Jing ^{[1
,2
]}

Guo, Ben-heng ^{[1
,3
]}

机构：

[1] Minist Sci & Technol, State Key Lab Dairy Biotechnol, Shanghai, Peoples R China

[2] Shanghai Engn Res Ctr Dairy Biotechnol, Shanghai, Peoples R China

[3] Bright Dairy & Food Co Ltd, Dairy Res Inst, Shanghai 200436, Peoples R China

来源：

FOODBORNE PATHOGENS AND DISEASE | 2015年 / 12卷 / 08期

关键词：

SOLID-PHASE EXTRACTION; BETA-LACTAM ANTIBIOTICS; BIODIESEL PRODUCTION; PESTICIDE-RESIDUES; SAMPLE TREATMENTS; VETERINARY DRUGS; BOVINE-MILK; MICROEXTRACTION; OPTIMIZATION; FLUORESCENCE;

D O I：

10.1089/fpd.2014.1916

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A precise and simplified method of sample preparation for the simultaneous quantification of the antibiotics beta-lactam, macrolide, tetracycline, sulfonamide, and quinolone in bovine milk was developed. The central composite design of response surface methodology was used to design and optimize the method for the determination of six different antibiotic residues in milk. The recovery of each antibiotic was studied using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. Octadecylsilane (C18), primary secondary amine (PSA), and sodium acetate (Na acetate) were the main factors affecting the recovery of each antibiotic. After optimization, the maximum predicted recovery rate was 84.18% for erythromycin under the optimized conditions of 101.20 mg C18, 52.00 mg PSA, and 1.01 g Na acetate. The recovery rates of the five other antibiotic residues ranged from 86.09% to 115.99%. The results suggested that modified QuEChERS could effectively be implemented in the analysis of antibiotic residues in milk.

引用

页码：693 / 703

页数：11

共 41 条

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