Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility

被引：8

作者：

Bartnik, Pawel ^{[1
,2
]}

Kacperczyk-Bartnik, Joanna ^{[1
,2
]}

Golawski, Ksawery ^{[3
]}

Sierdzinski, Janusz ^{[4
]}

Manka, Grzegorz ^{[5
]}

Kiecka, Mariusz ^{[5
]}

Lipa, Michal ^{[2
,3
]}

Warzecha, Damian ^{[3
]}

Spaczynski, Robert ^{[6
]}

Piekarski, Piotr ^{[6
]}

Banaszewska, Beata ^{[7
]}

Jakimiuk, Artur J. ^{[8
,9
]}

Issat, Tadeusz ^{[8
,10
]}

Rokita, Wojciech ^{[11
,12
]}

Mlodawski, Jakub ^{[11
,12
]}

Szubert, Maria ^{[2
,13
,14
]}

Sieroszewski, Piotr ^{[13
,15
]}

Raba, Grzegorz ^{[16
,17
]}

Szczupak, Kamil ^{[16
,17
]}

Kluz, Tomasz ^{[18
]}

Kluza, Marek ^{[18
]}

Czajkowski, Krzysztof ^{[1
]}

Wielgos, Miroslaw ^{[3
]}

Koc-Zorawska, Ewa ^{[19
,20
]}

Zorawski, Marcin ^{[21
]}

Laudanski, Piotr ^{[3
,22
]}

机构：

[1] Med Univ Warsaw, Dept Obstet & Gynecol 2, PL-00315 Warsaw, Poland

[2] Polish Soc Gynecologists & Obstetricians, Club 35, PL-53125 Wroclaw, Poland

[3] Med Univ Warsaw, Dept Obstet & Gynecol 1, PL-02015 Warsaw, Poland

[4] Med Univ Warsaw, Dept Med Informat & Telemed, PL-00581 Warsaw, Poland

[5] Angelius Provita Hosp, PL-40611 Katowice, Poland

[6] Poznan Univ Med Sci, Dept Gynecol Obstet & Gynecol Oncol, Div Infertil & Reprod Endocrinol, PL-61701 Poznan, Poland

[7] Poznan Univ Med Sci, Chair & Dept Lab Diagnost, PL-61701 Poznan, Poland

[8] Minist Interior, Cent Clin Hosp, Dept Obstet & Gynecol, PL-02507 Warsaw, Poland

[9] Inst Mother & Child Warsaw, Ctr Reprod Hlth, PL-01211 Warsaw, Poland

[10] Inst Mother & Child Warsaw, Dept Obstet & Gynecol, PL-01211 Warsaw, Poland

[11] Jan Kochanowski Univ Kielce, Coll Med, PL-25369 Kielce, Poland

[12] Prov Combined Hosp Kielce, Clin Obstet & Gynecol, PL-25736 Kielce, Poland

[13] Med Univ Lodz, Dept Gynecol & Obstet, PL-90419 Lodz, Poland

[14] Med Univ Lodz, Dept Surg Gynecol & Oncol, PL-90419 Lodz, Poland

[15] Med Univ Lodz, Dept Fetal Med & Gynecol, PL-90419 Lodz, Poland

[16] Clin Obstet & Gynecol Przemysl, PL-37700 Przemysl, Poland

[17] Univ Rzeszow, Dept Obstet & Gynecol, PL-35310 Rzeszow, Poland

[18] Med Coll Rzeszow Univ, Inst Med Sci, Dept Gynecol Gynecol Oncol & Obstet, PL-35310 Rzeszow, Poland

[19] Med Univ Bialystok, Dept Nephrol 2, PL-15089 Bialystok, Poland

[20] Med Univ Bialystok, Hypertens Dialysis Unit, PL-15089 Bialystok, Poland

[21] Med Univ Bialystok, Dept Clin Med, PL-15089 Bialystok, Poland

[22] OVIklin Infertil Ctr, PL-01377 Warsaw, Poland

来源：

BIOMEDICINES | 2022年 / 10卷 / 10期

关键词：

endometriosis; ELISA; epithelial-mesenchymal transition; infertility; peritoneal fluid; plasma; ZEB1; ZEB2; EPITHELIAL-MESENCHYMAL TRANSITION; EXPRESSION; CELLS; PATHOGENESIS; GENES; WOMEN; PROGRESSION; DISEASE; SNAIL; BETA;

D O I：

10.3390/biomedicines10102460

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc finger E-box-binding homeobox 1 (ZEB1) and zinc finger E-box-binding homeobox 2 (ZEB2) are transcription factors that regulate epithelial-mesenchymal transformation (EMT). The aim of this study was to compare levels of ZEB1 and ZEB2 in the peritoneal fluid and plasma between patients with and without endometriosis in order to assess their utility in the diagnostic process. Plasma and peritoneal fluid samples were collected from 50 patients with and 48 without endometriosis during planned surgical procedures in eight clinical centers. Quantitative ZEB1 and ZEB2 levels analyses were performed using a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). No significant differences were observed in ZEB1 levels in any of the subanalyses nor any differences regarding ZEB2 levels between patients with and without endometriosis. Plasma ZEB2 levels were significantly higher among patients with infertility compared to fertile women (16.07 +/- 12.70 ng/L vs. 12.07 +/- 11.92 ng/L; p < 0.04). Both ZEB1 and ZEB2 do not seem to have a significant value in the initial diagnosis of endometriosis as a single marker. The differences in ZEB2 plasma levels between patients with and without infertility indicate the possibility of EMT dysregulation in the pathogenesis of adverse fertility outcomes.

引用

页数：10

共 56 条

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