Transcriptional Silencing by Single-Stranded RNAs Targeting a Noncoding RNA That Overlaps a Gene Promoter

被引：18

作者：

Matsui, Masayuki ^{[1
,2
]}

Prakash, Thazha P. ^{[3
]}

Corey, David R. ^{[1
,2
]}

机构：

[1] Univ Texas SW Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA

[2] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

[3] ISIS Pharmaceut, Carlsbad, CA 92010 USA

来源：

ACS CHEMICAL BIOLOGY | 2013年 / 8卷 / 01期

基金：

美国国家卫生研究院;

关键词：

SIRNAS; ARGONAUTE2; CLEAVAGE;

D O I：

10.1021/cb300490j

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNAi using single-strand RNA would provide new options for therapeutic development and for investigating critical questions of mechanism. Using chemically modified single-strands, we test the hypothesis that single-stranded RNAs can engage the RNAi pathway and silence gene transcription. We find that a chemically modified single-stranded silencing RNA (ss-siRNA) designed to be complementary to a long noncoding RNA (IncRNA) requires argonaute protein, functions through the RNAi pathway, and inhibits gene transcription. These data expand the use of single-stranded RNA to cell nuclei.

引用

页码：122 / 126

页数：5