Multiple intravenous infusions of bone marrow mesenchymal stem cells reverse hyperglycemia in experimental type 2 diabetes rats

被引：106

作者：

Hao, Haojie ^{[1
]}

Liu, Jiejie ^{[1
]}

Shen, Jing ^{[2
]}

Zhao, Yali ^{[1
]}

Liu, Huilin ^{[1
]}

Hou, Qian ^{[1
]}

Tong, Chuan ^{[1
]}

Ti, Dongdong ^{[1
]}

Dong, Liang ^{[1
]}

Cheng, Yu ^{[2
]}

Mu, Yiming ^{[2
]}

Liu, Jianping ^{[2
,3
]}

Fu, Xiaobing ^{[1
]}

Han, Weidong ^{[1
]}

机构：

[1] Chinese Peoples Liberat Army Gen Hosp, Coll Life Sci, Inst Basic Med Sci, Beijing 100853, Peoples R China

[2] Chinese Peoples Liberat Army Gen Hosp, Dept Endocrinol, Beijing 100853, Peoples R China

[3] Jiangxi Prov Peoples Hosp, Dept Endocrinol, Nanchang, Jiangxi, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 436卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Bone marrow; Mesenchymal stem cells; Type; 2; diabetes; Multiple infusion; Hyperglycemia; INSULIN-RESISTANCE; STROMAL CELLS; MICE; INFLAMMATION; THERAPY; ISLETS; BLOOD;

D O I：

10.1016/j.bbrc.2013.05.117

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The worldwide rapid increase in diabetes poses a significant challenge to current therapeutic approaches. Single-dose mesenchymal stem cell (MSC) infusion ameliorates hyperglycemia but fails to restore normoglycemia in diabetic animals. We therefore hypothesized that multiple intravenous MSC infusions may reverse hyperglycemia in type 2 diabetes (T2D) rats. We administered serial allogenous bone-marrow derived MSC infusions (1 x 10(6) cells/infusion) via the tail vein once every 2 weeks to T2D rats, induced by high-fat diet and streptozocin (STZ) administration. Hyperglycemia decreased only transiently after a single infusion in early-phase (I week) T2D rats, but approximated normal levels after at least three-time infusions. This normal blood level was maintained for at least 9 weeks. Serum concentrations of both insulin and C-peptide were dramatically increased after serial MSC infusions. Oral glucose tolerance tests revealed that glucose metabolism was significantly ameliorated. Immunofluorescence analysis of insulin/glucagon staining revealed the restoration of islet structure and number after multiple MSC treatments. When multiple-MSC treatment was initiated in late-phase (5 week) T2D rats, the results were slightly different. The results of this study suggested that a multiple-MSC infusion strategy offers a viable clinical option for T2D patients. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：418 / 423

页数：6

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