CD99 plays a major role in the migration of monocytes through endothelial junctions

被引:375
作者
Schenkel, AR [1 ]
Mamdouh, Z [1 ]
Chen, X [1 ]
Liebman, RM [1 ]
Muller, WA [1 ]
机构
[1] Cornell Univ, Weill Med Coll, Dept Pathol, New York, NY 10021 USA
关键词
D O I
10.1038/ni749
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CD99 is a heavily O-glycosylated 32-kD type I transmembrane protein that is expressed on most hematopoietic cells. We show here that CD99 is expressed on endothelial cells and is concentrated at the borders between confluent cells. We found that a monoclonal antibody to CD99, hec2, selectively inhibited diapedesis of monocytes across endothelial cells by >90%. Diapedesis involved the homophilic interaction of CD99 on monocytes with CD99 on endothelial junctions. CD99 functioned distally to the point at which platelet-endothelial cell adhesion molecule 1 (PECAM-1, also known as CD31), another adhesion molecule involved in transmigration, played its critical role. Confocal microscopy showed that anti-PECAM-1 arrested leukocytes on the apical surface of endothelium, whereas blocking CD99 arrested monocytes at a point where they were partially through the junction. Therefore, diapedesis, the forward migration of leukocytes through endothelial junctions, is regulated sequentially by two distinct molecules, PECAM-1 and CD99.
引用
收藏
页码:143 / 150
页数:8
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