Protective efficacy of a DNA vaccine encoding capsid protein of porcine circovirus-like virus P1 against porcine circovirus 2 in mice

被引：5
作者：
Zhang, Dan [1 ,2 ]
He, Kongwang [1 ,3 ]
Wen, Libin [1 ,3 ]
Fan, Hongjie [2 ]
机构：
[1] Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210000, Jiangsu, Peoples R China
[2] Nanjing Agr Univ, Coll Vet Med, Nanjing 210014, Jiangsu, Peoples R China
[3] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China
来源：
MICROBIOLOGY AND IMMUNOLOGY | 2018年 / 62卷 / 03期
基金：
中国国家自然科学基金;
关键词：
DNA vaccine; mice; porcine circovirus 2; porcine circovirus-like virus P1; MULTISYSTEMIC WASTING SYNDROME; TYPE-2; IMMUNOGENICITY; EXPRESSION; PIGS;
D O I：
10.1111/1348-0421.12571
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The capsid protein is the major immunogenic protein of porcine circovirus 2 (PCV2). The nucleotide sequence of porcine circovirus-like virus P1 shares high homology with open reading frame (ORF) 2 of PCV2, and ORF1 of P1 encodes its structural protein. Mice were vaccinated twice intramuscularly with a plasmid expressing the P1 ORF1 protein (pcDNA3.1(+)-ORF1) at 2-week intervals. All animals vaccinated with pcDNA3.1(+)-ORF1 developed higher specific anti-P1 antibody levels, and had less PCV2 viremia and milder histopathological changes than PCV2-challenged mice in the control group. Our results show that the P1 DNA vaccine elicited immune responses against PCV2 infection in a mouse model.
引用
收藏
页码：195 / 199
页数：5
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