Genetic Identification and Antimicrobial Activity of Streptomyces sp. Strain Je 1-6 Isolated from Rhizosphere Soil of Juniperus excelsa Bieb

Actinobacteria isolated from poorly explored biotopes are of interest as producers of biologically active compounds. A report is given about the producer of lydicamycin Je 1-6 isolated from the rhizosphere soil of J. excelsa Bieb. collected from the Crimean Peninsula, Ukraine, that demonstrated its antagonistic activity against gram-positive bacteria. Based on the 16S rRNA gene sequence analysis, the strain Je 1-6 was affiliated to the Streptomyces genus. The crude extract from the biomass of this strain showed its activity against gram-positive bacteria, including polyresistant clinical isolates of Staphylococcus sp. The dereplication analysis of the extract of the strain Je 1-6 revealed lydicamycin and its congeners: 30-demethyllydicamycin, 30-demethyl-8-deoxylydicamycin, and 8-deoxylydicamycin. MLSA analysis based on the 16S rRNA gene and five housekeeping genes atpD, gyrB, rpoB, recA, and trpB of the strain Je 1-6 showed a high level of homology with Streptomyces sp. ID38640.