Enzymatic transesterification of Jatropha oil

被引：145

作者：

Kumari, Annapurna ^{[1
]}

Mahapatra, Paramita ^{[1
]}

Garlapati, Vijay Kumar ^{[1
]}

Banerjee, Rintu ^{[1
]}

机构：

[1] Indian Inst Technol, Agr & Food Engn Dept, Kharagpur 721302, W Bengal, India

来源：

BIOTECHNOLOGY FOR BIOFUELS | 2009年 / 2卷

关键词：

BIODIESEL FUEL PRODUCTION; LIPASE-CATALYZED TRANSESTERIFICATION; CANDIDA-ANTARCTICA LIPASE; IMMOBILIZED-LIPASE; OLEATE SYNTHESIS; RHIZOPUS-ORYZAE; VEGETABLE-OIL; SOLVENT; WATER; TRIGLYCERIDES;

D O I：

10.1186/1754-6834-2-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Transesterification of Jatropha oil was carried out in t-butanol solvent using immobilized lipase from Enterobacter aerogenes. The presence of t-butanol significantly reduced the negative effects caused by both methanol and glycerol. The effects of various reaction parameters on transesterification of Jatropha oil were studied. Results: The maximum yield of biodiesel was 94% (of which 68% conversion was achieved with respect to methyl oleate) with an oil: methanol molar ratio of 1: 4, 50 U of immobilized lipase/g of oil, and a t-butanol: oil volume ratio of 0.8:1 at 55 degrees C after 48 h of reaction time. There was negligible loss in lipase activity even after repeated use for seven cycles. Conclusion: To the best of our knowledge this is the first report on biodiesel synthesis using immobilized E. aerogenes lipase.

引用

页数：7

共 42 条

[11] A concept for simultaneous wasteland reclamation, fuel production, and socio-economic development in degraded areas in India:: Need, potential and perspectives of Jatropha plantations [J].

Francis, G ;

Edinger, R ;

Becker, K .

NATURAL RESOURCES FORUM, 2005, 29 (01) :12-24

[12] Biodiesel fuel production by transesterification of oils [J].

Fukuda, H. (fukuda@cx.kobe-u.ac.jp), 2001, Society of Fermentation and Bioengineering, Japan (92)

[13] 1-butyl oleate synthesis by immobilized lipase from Rhizopus oryzae:: a comparative study between n-hexane and solvent-free system [J].

Ghamgui, H ;

Karra-Chaâbouni, M ;

Gargouri, Y .

ENZYME AND MICROBIAL TECHNOLOGY, 2004, 35 (04) :355-363

[14] Immobilized lipase-catalysed production of alkyl esters of restaurant grease as biodiesel [J].

Hsu, AF ;

Jones, K ;

Foglia, TA ;

Marmer, WN .

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2002, 36 :181-186

[15] Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase [J].

Iso, M ;

Chen, BX ;

Eguchi, M ;

Kudo, T ;

Shrestha, S .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 16 (01) :53-58

[16] Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent [J].

Kaieda, M ;

Samukawa, T ;

Matsumoto, T ;

Ban, K ;

Kondo, A ;

Shimada, Y ;

Noda, H ;

Nomoto, F ;

Ohtsuka, K ;

Izumoto, E ;

Fukuda, H .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (06) :627-631

[17] Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system [J].

Kaieda, M ;

Samukawa, T ;

Kondo, A ;

Fukuda, H .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2001, 91 (01) :12-15

[18] Lipase catalyzed methanolysis of vegetable oils in aqueous medium by Cryptococcus spp. S-2 [J].

Kamini, NR ;

Iefuji, H .

PROCESS BIOCHEMISTRY, 2001, 37 (04) :405-410

[19] EXTRACELLULAR LIPASE OF PSEUDOMONAS SP STRAIN ATCC-21808 - PURIFICATION, CHARACTERIZATION, CRYSTALLIZATION, AND PRELIMINARY-X-RAY DIFFRACTION DATA [J].

KORDEL, M ;

HOFMANN, B ;

SCHOMBURG, D ;

SCHMID, RD .

JOURNAL OF BACTERIOLOGY, 1991, 173 (15) :4836-4841

[20] Immobilized Candida antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium [J].

Köse, Ö ;

Tüter, M ;

Aksoy, HA .

BIORESOURCE TECHNOLOGY, 2002, 83 (02) :125-129

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