Enzymatic transesterification of Jatropha oil

被引：145

作者：

Kumari, Annapurna ^{[1
]}

Mahapatra, Paramita ^{[1
]}

Garlapati, Vijay Kumar ^{[1
]}

Banerjee, Rintu ^{[1
]}

机构：

[1] Indian Inst Technol, Agr & Food Engn Dept, Kharagpur 721302, W Bengal, India

来源：

BIOTECHNOLOGY FOR BIOFUELS | 2009年 / 2卷

关键词：

BIODIESEL FUEL PRODUCTION; LIPASE-CATALYZED TRANSESTERIFICATION; CANDIDA-ANTARCTICA LIPASE; IMMOBILIZED-LIPASE; OLEATE SYNTHESIS; RHIZOPUS-ORYZAE; VEGETABLE-OIL; SOLVENT; WATER; TRIGLYCERIDES;

D O I：

10.1186/1754-6834-2-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Transesterification of Jatropha oil was carried out in t-butanol solvent using immobilized lipase from Enterobacter aerogenes. The presence of t-butanol significantly reduced the negative effects caused by both methanol and glycerol. The effects of various reaction parameters on transesterification of Jatropha oil were studied. Results: The maximum yield of biodiesel was 94% (of which 68% conversion was achieved with respect to methyl oleate) with an oil: methanol molar ratio of 1: 4, 50 U of immobilized lipase/g of oil, and a t-butanol: oil volume ratio of 0.8:1 at 55 degrees C after 48 h of reaction time. There was negligible loss in lipase activity even after repeated use for seven cycles. Conclusion: To the best of our knowledge this is the first report on biodiesel synthesis using immobilized E. aerogenes lipase.

引用

页数：7

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