High resolution two-dimensional electrophoresis as a tool to differentiate wild from farmed cod (Gadus morhua) and to assess the protein composition of klipfish

被引：25

作者：

Martinez, Iciar ^{[1
]}

Slizyte, Rasa ^{[1
]}

Dauksas, Egidijus ^{[1
]}

机构：

[1] SINTEF, Dept Proc Technol, NO-7465 Trondheim, Norway

来源：

FOOD CHEMISTRY | 2007年 / 102卷 / 02期

关键词：

wild cod; farmed cod; klipfish; fish muscle; myofibrillar proteins; two-dimensional electrophoresis;

D O I：

10.1016/j.foodchem.2006.03.037

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Tris and CHAPS-urea extracts from wild and farmed cod muscle and from rehydrated cod klipfish fillets were analyzed by one (1DE) and two-dimensional electrophoresis (2DE). 2DE maps of tris extracts from farmed cod differed from the wild in a series of spots of Mw 35 and 45 kDa. The CHAPS-urea extracts from farmed cod had a several spots of Mw between 100 and 45 kDa, which were hardly detectable in wild cod and very prominent in klipfish. Klipfish was clearly different from the other samples: the myosin heavy chain was hardly detectable in these samples, and the tris extracts contained fewer, and the CHAPS-urea more spots than the corresponding extracts from the raw muscles. Further identification of these potentially diagnostic spots will make it easier the differentiation of farmed from wild cod and the evaluation of klipfish processing on the protein content of the product. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：504 / 510

页数：7

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