Anti-hepatoma activity of taxol in vitro

被引：0

作者：

Yuan, JH

Wang, XW

Luo, D

Xie, Y

Xie, H ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Hong Kong, Hong Kong, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China

来源：

ACTA PHARMACOLOGICA SINICA | 2000年 / 21卷 / 05期

关键词：

taxol; hepatocellular carcinoma; cultured tumor cells; cell division; cell cycle; apoptosis;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To investigate the in vitro anti-hepatoma activity of taxol against SMMC-7721 human hepatoma cells. METHODS: The hepatoma cell growth was measured by MTT-microculture tetrazolium assay. Cell-cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic examination. RESULTS: Taxol inhibited the hepatoma cell growth in concentration- and time-dependent manners with IC50 Of 18.96 nmol.L-1. Marked cell accumulation in G(2)/M phase and multinucleated cells were also observed after treatment with taxol 10 nmol.L-1. In addition, taxol at 10 nmol.L-1 could induce the apoptosis of hepatoma cells. CONCLUSION: Taxol suppresses the growth of SMMC-7721 human hepatoma cells in vitro by causing cell-cycle arrest, aberrant mitosis, and apoptosis of the human hepatoma cells.

引用

页码：450 / 454

页数：5

共 24 条

[1] MICROTUBULE DEPENDENCY OF P34(CDC2) INACTIVATION AND MITOTIC EXIT IN MAMMALIAN-CELLS [J].