Modulation of TGF-beta 1 production from human keratinocytes by UVB

被引：59

作者：

Lee, HST ^{[1
]}

Kooshesh, F ^{[1
]}

Sauder, DN ^{[1
]}

Kondo, S ^{[1
]}

机构：

[1] UNIV TORONTO,SUNNYBROOK HLTH SCI CTR,DIV DERMATOL,TORONTO,ON,CANADA

来源：

EXPERIMENTAL DERMATOLOGY | 1997年 / 6卷 / 02期

关键词：

inflammation; immunosuppression; cytokine; photobiology;

D O I：

10.1111/j.1600-0625.1997.tb00155.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Transforming growth factor-beta (TGF-beta) plays an important role not only in cell growth control but also in inflammation and immunoregulation. There are at least five different isoforms of TGF-beta. TGF-beta 1 has a large variety of biological functions including the modulation of inflammation and the immune system and has most extensively been studied in skin. Since ultraviolet B (UVB) is known to induce skin erythema and immunosuppression, we sought to examine whether UVB would alter the expression and production of TGF-beta 1 in normal human keratinocytes. Using reverse transcription-polymerase chain reaction (RT-PCR), constitutive expression of TGF-beta 1 mRNA was detected in keratinocytes and the level of TGF-beta 1 mRNA was increased 4 and 8 h after 300 J/m(2) UVB irradiation. Production of TGF-beta 1 protein in culture supernatants assayed by ELISA was also increased at 24 h after irradiation. Cycloheximide treatment blocked this TGF-beta 1 protein induction indicating de novo protein synthesis of TGF-beta 1 from keratinocytes induced by UVB. These results suggest a possible role for TGF-beta 1 in UVB-induced skin inflammation and immunosuppression.

引用

页码：105 / 110

页数：6

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