Circular RNA expression alterations in extracellular vesicles isolated from murine heart post ischemia/reperfusion injury

被引:49
作者
Ge, Xinyu [1 ,2 ,3 ,4 ]
Meng, Qingshu [1 ,2 ,3 ]
Zhuang, Rulin [1 ,2 ,3 ,4 ]
Yuan, Dongsheng [1 ,2 ,3 ,4 ]
Liu, Jing [1 ,2 ,3 ,4 ]
Lin, Fang [1 ,2 ,3 ]
Fan, Huimin [1 ,2 ,3 ,4 ,5 ]
Zhou, Xiaohui [1 ,2 ,3 ]
机构
[1] Tongji Univ, Sch Med, Shanghai East Hosp, Res Ctr Translat Med, Shanghai 200120, Peoples R China
[2] Tongji Univ, Sch Med, Shanghai East Hosp, Shanghai Heart Failure Res Ctr, Shanghai 200120, Peoples R China
[3] Tongji Univ, Sch Med, Inst Integrated Tradit Chinese & Western Med Card, Shanghai 200120, Peoples R China
[4] Tongji Univ, Sch Med, Shanghai East Hosp, Dept Cardiovasc Surg, Shanghai 200120, Peoples R China
[5] Tongji Univ, Sch Med, Shanghai East Hosp, Dept Heart Failure, Shanghai 200120, Peoples R China
基金
中国国家自然科学基金;
关键词
Ischemia/reperfusion injury; Extracellular vesicles; Circular RNA; MicroRNA;
D O I
10.1016/j.ijcard.2019.08.024
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Increasing studies indicated the involvement of extracellular vesicles (EVs) in cardiovascular diseases. However, the role of circular RNAs (circRNAs) in cardiac EVs (cEVs) during ischemia/reperfusion (I/R) injury remain unclear. Methods: We isolated the cEVs from I/R injured hearts and performed RNA sequencing (RNA-seq) to identify the profile of circRNA in cEVs and investigated their potential roles in I/R pathological process. Results: Cardiac I/R induced a significantly elevated release of EVs in heart within 24 h. RNA-seq of cEVs identified 185 significantly differentially expressed (DE) circRNAs including 119 down-regulated and 66 up-regulated circRNAs in I/R group compared with the sham. GO and pathway analysis showed that these DE-circRNAs were associated with protein binding and kinase activator activity and mainly involved in the metabolic process. The circRNA-miRNA analysis exhibited the broad potentials of the DE-circRNAs to regulate target genes by acting on the miRNAs. Conclusions: These findings revealed for the first time the specific expression pattern of circRNAs in EVs derived from sham and I/R heart tissues and provided some potential targets and pathways involving in I/R injury which may provide important evidences for the role of both circRNA and EVs in the pathology of cardiac I/R. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:136 / 140
页数:5
相关论文
共 14 条
[1]   Circular RNA in cardiovascular disease [J].
Altesha, M-Ashraf ;
Ni, Tiffany ;
Khan, Afaan ;
Liu, Kexiang ;
Zheng, Xiufen .
JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (05) :5588-5600
[2]   Cardiosome mediated regulation of MMP9 in diabetic heart: role of mir29b and mir455 in exercise [J].
Chaturvedi, Pankaj ;
Kalani, Anuradha ;
Medina, Ilza ;
Familtseva, Anastasia ;
Tyagi, Suresh C. .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2015, 19 (09) :2153-2161
[3]   Eph/Ephrin Signaling in Injury and Inflammation [J].
Coulthard, Mark G. ;
Morgan, Michael ;
Woodruff, Trent M. ;
Arumugam, Thiruma V. ;
Taylor, Stephen M. ;
Carpenter, Todd C. ;
Lackmann, Martin ;
Boyd, Andrew W. .
AMERICAN JOURNAL OF PATHOLOGY, 2012, 181 (05) :1493-1503
[4]   Exosomal circRNA_100284 from arsenite-transformed cells, via microRNA-217 regulation of EZH2, is involved in the malignant transformation of human hepatic cells by accelerating the cell cycle and promoting cell proliferation [J].
Dai, Xiangyu ;
Chen, Chao ;
Yang, Qianlei ;
Xue, Junchao ;
Chen, Xiong ;
Sun, Baofei ;
Luo, Fei ;
Liu, Xinlu ;
Xiao, Tian ;
Xu, Hui ;
Sun, Qian ;
Zhang, Aihua ;
Liu, Qizhan .
CELL DEATH & DISEASE, 2018, 9
[5]   Circular RNAs: Identification, biogenesis and function [J].
Ebbesen, Karoline K. ;
Kjems, Jorgen ;
Hansen, Thomas B. .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2016, 1859 (01) :163-168
[6]   Circular RNA and its mechanisms in disease: From the bench to the clinic [J].
Han, Bing ;
Chao, Jie ;
Yao, Honghong .
PHARMACOLOGY & THERAPEUTICS, 2018, 187 :31-44
[7]   Fibroblast-specific TGF-β-Smad2/3 signaling underlies cardiac fibrosis [J].
Khalil, Hadi ;
Kanisicak, Onur ;
Prasad, Vikram ;
Correll, Robert N. ;
Fu, Xing ;
Schips, Tobias ;
Vagnozzi, Ronald J. ;
Liu, Ruijie ;
Thanh Huynh ;
Lee, Se-Jin ;
Karch, Jason ;
Molkentin, Jeffery D. .
JOURNAL OF CLINICAL INVESTIGATION, 2017, 127 (10) :3770-3783
[8]   Circular RNA is enriched and stable in exosomes: a promising biomarker for cancer diagnosis [J].
Li, Yan ;
Zheng, Qiupeng ;
Bao, Chunyang ;
Li, Shuyi ;
Guo, Weijie ;
Zhao, Jiang ;
Chen, Di ;
Gu, Jianren ;
He, Xianghuo ;
Huang, Shenglin .
CELL RESEARCH, 2015, 25 (08) :981-984
[9]   Intra-Cardiac Release of Extracellular Vesicles Shapes Inflammation Following Myocardial Infarction [J].
Loyer, Xavier ;
Zlatanova, Ivana ;
Devue, Cecile ;
Yin, Min ;
Howangyin, Kiave-Yune ;
Klaihmon, Phatchanat ;
Guerin, Coralie L. ;
Kheloufi, Marouane ;
Vilar, Jose ;
Zannis, Konstantinos ;
Fleischmann, Bernd K. ;
Hwang, Do Won ;
Park, Jongmin ;
Lee, Hakho ;
Menasche, Philippe ;
Silvestre, Jean-Sebastien ;
Boulanger, Chantal M. .
CIRCULATION RESEARCH, 2018, 123 (01) :100-106
[10]   Myocardial ischemia/reperfusion-injury, a clinical view on a complex pathophysiological process [J].
Moens, AL ;
Claeys, MJ ;
Timmermans, JP ;
Vrints, CJ .
INTERNATIONAL JOURNAL OF CARDIOLOGY, 2005, 100 (02) :179-190