Neurotrophin-4 (NT-4) and glial cell line-derived neurotrophic factor (GDNF) promote the survival of corticospinal motor neurons of neonatal rats in vitro

被引：27
作者：
Junger, H
Varon, S
机构：
[1] Department of Biology, Univ. California, S. Diego, 9500 G., San Diego
来源：
BRAIN RESEARCH | 1997年 / 762卷 / 1-2期
关键词：
corticospinal motor neuron; ciliary neurotrophic factor; glial cell line-derived neurotrophic factor; neurotrophins; cell survival; cell culture;
D O I：
10.1016/S0006-8993(97)00347-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We have used enriched dissociated, low density cultures of neonatal rat corticospinal motor neurons to evaluate the survival-promoting effect of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), and glial cell line-derived neurotrophic factor (GDNF) and the ciliary neurotrophic factor (CNTF). Our current findings demonstrated that CNTF promoted the survival of corticospinal motor neurons, in the same fashion and at an equivalent potency, as was previously described using a different assay system. Among the other factors tested, we also found that NT-4 and GDNF increased the number of surviving neurons in a dose-dependent manner, whereas NGF, BDNF and NT-3 showed no survival promoting effect on corticospinal motor neurons. (C) 1997 Elsevier Science B.V.
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收藏
页码：56 / 60
页数：5
相关论文
共 25 条
[11]   RESCUE OF ADULT-MOUSE MOTONEURONS FROM INJURY-INDUCED CELL-DEATH BY GLIAL-CELL LINE-DERIVED NEUROTROPHIC FACTOR [J].
LI, LX ;
WU, WT ;
LIN, LFH ;
LEI, M ;
OPPENHEIM, RW ;
HOUENOU, LJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (21) :9771-9775
[12]   CNTF PROMOTES THE SURVIVAL OF NEONATAL RAT CORTICOSPINAL NEURONS INVITRO [J].
MAGAL, E ;
LOUIS, JC ;
OUDEGA, M ;
VARON, S .
NEUROREPORT, 1993, 4 (06) :779-782
[13]   MOLECULAR-CLONING OF RAT TRKC AND DISTRIBUTION OF CELLS EXPRESSING MESSENGER-RNAS FOR MEMBERS OF THE TRK FAMILY IN THE RAT CENTRAL-NERVOUS-SYSTEM [J].
MERLIO, JP ;
ERNFORS, P ;
JABER, M ;
PERSSON, H .
NEUROSCIENCE, 1992, 51 (03) :513-532
[14]   DEVELOPING MOTOR-NEURONS RESCUED FROM PROGRAMMED AND AXOTOMY-INDUCED CELL-DEATH BY GDNF [J].
OPPENHEIM, RW ;
HOUENOU, LJ ;
JOHNSON, JE ;
LIN, LFH ;
LI, LX ;
LO, AC ;
NEWSOME, AL ;
PREVETTE, DM ;
WANG, SW .
NATURE, 1995, 373 (6512) :344-346
[15]   DISTRIBUTION OF CORTICOSPINAL MOTOR-NEURONS IN THE POSTNATAL RAT - QUANTITATIVE EVIDENCE FOR MASSIVE COLLATERAL ELIMINATION AND MODEST CELL-DEATH [J].
OUDEGA, M ;
VARON, S ;
HAGG, T .
JOURNAL OF COMPARATIVE NEUROLOGY, 1994, 347 (01) :115-126
[16]  
Schacht C. M., 1996, Society for Neuroscience Abstracts, V22, P1952
[17]   NEUROTROPHIN-4/5 POSTPONES THE DEATH OF INJURED SPINAL MOTONEURONS IN NEWBORN RATS [J].
SCHMALBRUCH, H ;
ROSENTHAL, A .
BRAIN RESEARCH, 1995, 700 (1-2) :254-260
[18]   NEUROTROPHIN-3 ENHANCES SPROUTING OF CORTICOSPINAL TRACT DURING DEVELOPMENT AND AFTER ADULT SPINAL-CORD LESION [J].
SCHNELL, L ;
SCHNEIDER, R ;
KOLBECK, R ;
BARDE, YA ;
SCHWAB, ME .
NATURE, 1994, 367 (6459) :170-173
[19]   EXPRESSION OF GDNF MESSENGER-RNA IN RAT AND HUMAN NERVOUS-TISSUE [J].
SPRINGER, JE ;
MU, X ;
BERGMANN, LW ;
TROJANOWSKI, JQ .
EXPERIMENTAL NEUROLOGY, 1994, 127 (02) :167-170
[20]   THE DEVELOPMENT OF THE CORTICOSPINAL PROJECTION [J].
STANFIELD, BB .
PROGRESS IN NEUROBIOLOGY, 1992, 38 (02) :169-202
123