MAPK-dependent expression of p21WAF and p27kip1 in PMA-induced differentiation of HL60 cells

被引：94
作者：
Das, D
Pintucci, G
Stern, A
机构：
[1] NYU, Sch Med, Dept Pharmacol, New York, NY 10016 USA
[2] NYU, Sch Med, Dept Surg, New York, NY USA
来源：
FEBS LETTERS | 2000年 / 472卷 / 01期
关键词：
differentiation; mitogen-activated protein kinase; p21(WAF); p27(kip1);
D O I：
10.1016/S0014-5793(00)01416-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Treatment of HL60 cells with phorbol 12-myristate 13-acetate (PMA) results in growth arrest and differentiation ton ards the macrophage lineage. PMA-induced changes are easily monitored by morphological changes while cells in suspension start adhering onto substrate, PMA induces rapid activation of the extracellular signal-regulated kinases (ERKs), Activation of the ERK pathway is essential to PMA-induced differentiation of HL60 cells. PMA also induces the expression of the cyclin-dependent kinase inhibitors p21(WAF) and p27(kip1) which is modulated by the use of an inhibitor of the ER cascade. This implies that a Link exists between ERK activation and p21(WAF) and p27(kip1) induction in the process of terminal differentiation. (C) 2000 Federation of European Biochemical Societies.
引用
收藏
页码：50 / 52
页数：3
相关论文
共 13 条
[1]  
AHN NG, 1990, J BIOL CHEM, V265, P11487
[2]   WAF1, A POTENTIAL MEDIATOR OF P53 TUMOR SUPPRESSION [J].
ELDEIRY, WS ;
TOKINO, T ;
VELCULESCU, VE ;
LEVY, DB ;
PARSONS, R ;
TRENT, JM ;
LIN, D ;
MERCER, WE ;
KINZLER, KW ;
VOGELSTEIN, B .
CELL, 1993, 75 (04) :817-825
[3]   THE BETA-PDGF RECEPTOR INDUCES NEURONAL DIFFERENTIATION OF PC12 CELLS [J].
HEASLEY, LE ;
JOHNSON, GL .
MOLECULAR BIOLOGY OF THE CELL, 1992, 3 (05) :545-553
[4]  
Liu Y, 1996, CANCER RES, V56, P31
[5]  
MICHIELI P, 1994, CANCER RES, V54, P3391
[6]  
MIYASAKA T, 1990, J BIOL CHEM, V265, P4730
[7]   Inhibitors of cyclin-dependent kinases promote survival of post-mitotic neuronally differentiated PC12 cells and sympathetic neurons [J].
Park, DS ;
Farinelli, SE ;
Greene, LA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (14) :8161-8169
[8]   P53-INDEPENDENT EXPRESSION OF P21(CIP)1 IN MUSCLE AND OTHER TERMINALLY DIFFERENTIATING CELLS [J].
PARKER, SB ;
EICHELE, G ;
ZHANG, PM ;
RAWLS, A ;
SANDS, AT ;
BRADLEY, A ;
OLSON, EN ;
HARPER, JW ;
ELLEDGE, SJ .
SCIENCE, 1995, 267 (5200) :1024-1027
[9]   A novel, nerve growth factor-activated pathway involving nitric oxide, p53, and p21(WAF1) regulates neuronal differentiation of PC12 cells [J].
Poluha, W ;
Schonhoff, CM ;
Harrington, KS ;
Lachyankar, MB ;
Crosbie, NE ;
Bulseco, DA ;
Ross, AH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (38) :24002-24007
[10]   Cell cycle arrest mediated by the MEK/mitogen-activated protein kinase pathway [J].
Pumiglia, KM ;
Decker, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (02) :448-452
12