Top-and-side dual-view microfluidic device with embedded prism

被引：8
作者：
Kazama, Yuto [1 ]
Carlen, Edwin T. [2 ,3 ]
van den Berg, Albert [3 ]
Hibara, Akihide [4 ,5 ]
机构：
[1] Univ Tokyo, Inst Ind Sci, Tokyo, Japan
[2] Univ Tsukuba, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki, Japan
[3] Univ Twente, MESA, Enschede, Netherlands
[4] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Sendai, Miyagi, Japan
[5] Tokyo Inst Technol, Grad Sch Sci, Dept Chem, Tokyo, Japan
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 248卷
关键词：
Dual view; Microfluidic channel; PDMS; Dark-field view; Fluorescence; TOTAL ANALYSIS SYSTEMS; SPONTANEOUS EMULSIFICATION; DROPLET MICROFLUIDICS; CELLS; TECHNOLOGY; FLOW; CHIP;
D O I：
10.1016/j.snb.2017.04.013
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A polydimethylsiloxane microfluidic device enabling dual-view visualization is proposed and demonstrated. A prism with a 2 mm square base was embedded beside a 300 mu m-wide microchannel. In addition to ordinary visualization from the top of the device, the microchannel could be viewed from the side, and its optical path was reflected to the top by the prism. The top and side dual visualization in a single field of view was then realized with a single objective lens. The shifts in the focal point in the top and side directions were modeled, and a compensation method utilizing a flat sheet was used. After simultaneous bright-field and dark-field visualization was attained, dual-view fluorescence imaging of the fluorescent solution and cells was realized. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：753 / 760
页数：8
相关论文
共 40 条
[1]   Microfluidic devices for cellomics: a review [J].
Andersson, H ;
van den Berg, A .
SENSORS AND ACTUATORS B-CHEMICAL, 2003, 92 (03) :315-325
[2]   Micro total analysis systems. 2. Analytical standard operations and applications [J].
Auroux, PA ;
Iossifidis, D ;
Reyes, DR ;
Manz, A .
ANALYTICAL CHEMISTRY, 2002, 74 (12) :2637-2652
[3]   Micro- and nanoscale deformation measurement of surface and internal planes via digital image correlation [J].
Berfield, T. A. ;
Patel, J. K. ;
Shimmin, R. G. ;
Braun, P. V. ;
Lambros, J. ;
Sottos, N. R. .
EXPERIMENTAL MECHANICS, 2007, 47 (01) :51-62
[4]   Imaging adherent cells in the microfluidic channel hidden by flowing RBCs as occluding objects by a holographic method [J].
Bianco, Vittorio ;
Merola, Francesco ;
Miccio, Lisa ;
Memmolo, Pasquale ;
Gennari, Oriella ;
Paturzo, Melania ;
Netti, Paolo Antonio ;
Ferraro, Pietro .
LAB ON A CHIP, 2014, 14 (14) :2499-2504
[5]   PDMS 2D optical lens integrated with microfluidic channels: principle and characterization [J].
Camou, S ;
Fujita, H ;
Fujii, T .
LAB ON A CHIP, 2003, 3 (01) :40-45
[6]   An integrated fluorescence detection system in poly(dimethylsiloxane) for microfluidic applications [J].
Chabinyc, ML ;
Chiu, DT ;
McDonald, JC ;
Stroock, AD ;
Christian, JF ;
Karger, AM ;
Whitesides, GM .
ANALYTICAL CHEMISTRY, 2001, 73 (18) :4491-4498
[7]   Compact and modular multicolour fluorescence detector for droplet microfluidics [J].
Cole, Russell H. ;
de Lange, Niek ;
Gartner, Zev J. ;
Abate, Adam R. .
LAB ON A CHIP, 2015, 15 (13) :2754-2758
[8]   Micro Total Analysis Systems: Fundamental Advances and Biological Applications [J].
Culbertson, Christopher T. ;
Mickleburgh, Tom G. ;
Stewart-James, Samantha A. ;
Sellens, Kathleen A. ;
Pressnall, Melissa .
ANALYTICAL CHEMISTRY, 2014, 86 (01) :95-118
[9]   Coplanar electrowetting-induced stirring as a tool to manipulate biological samples in lubricated digital microfluidics. Impact of ambient phase on drop internal flow pattern [J].
Davoust, Laurent ;
Fouillet, Yves ;
Malk, Rachid ;
Theisen, Johannes .
BIOMICROFLUIDICS, 2013, 7 (04)
[10]   Inertial microfluidics [J].
Di Carlo, Dino .
LAB ON A CHIP, 2009, 9 (21) :3038-3046
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