Characterization of anti-human interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) monoclonal antibodies and their application in the measurement of human IL-18 by ELISA

被引:120
作者
Taniguchi, M [1 ]
Nagaoka, K [1 ]
Kunikata, T [1 ]
Kayano, T [1 ]
Yamauchi, H [1 ]
Nakamura, S [1 ]
Ikeda, M [1 ]
Orita, K [1 ]
Kurimoto, M [1 ]
机构
[1] HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR,OKAYAMA 702,JAPAN
关键词
IL-18; IGIF; ELISA; monoclonal antibody; cytokine;
D O I
10.1016/S0022-1759(97)00094-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) is a novel cytokine, which is a potent inducer of IFN-gamma production and plays an important role in Th1 responses. In order to develop a specific ELISA for the measurement of human IL-18, we established 13 anti-human IL-18 monoclonal antibodies and characterized them. 7 murine anti-human IL-18 mAbs and 6 rat anti-human IL-18 mAbs were obtained by fusion of splenocytes from mice or rats immunized with human IL-18, with SP2/0 myeloma cells, These antibodies were classified into 4 groups according to competitive binding ELISAs to the human IL-18 molecule, 1 murine mAb and all 6 rat mAbs neutralized IFN-gamma production induced by IL-ls. A specific human IL-18 ELISA was developed using two neutralizing mAbs (#125-2B and #159-12B). This ELISA detects human IL-18 with a minimum detection limit of 10 pg/ml, but does not react with heat-denatured human IL-18. The ELISA does not show any cross-reactivity with other cytokines. Using this assay, human IL-18 was measurable in the plasma of leukemia patients. This ELISA would become a powerful tool for investigating the relationship between IL-18 and various diseases or analyzing the control mechanisms of IL-18 production from IL-18 producing cells. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:107 / 113
页数:7
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