Molecular mapping of a novel blast resistance gene Pi38 in rice using SSLP and AFLP markers

被引：46
作者：
Gowda, M.
Roy-Barman, S.
Chattoo, B. B. [1 ]
机构：
[1] Maharaja Sayajirao Univ Baroda, Fac Sci, Dept Microbiol, Baroda 390002, Gujarat, India
[2] Maharaja Sayajirao Univ Baroda, Fac Sci, Ctr Biotechnol, Baroda 390002, Gujarat, India
来源：
PLANT BREEDING | 2006年 / 125卷 / 06期
关键词：
Orvza sativa; Magnaporthe grisea; blast resistance gene; Pi38; 'Tadukan';
D O I：
10.1111/j.1439-0523.2006.01248.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Blast, caused by Magnaporthe grisea, is the most devastating disease of rice worldwide. In this study, the main objective was to identify and map a new gene for blast resistance, in an indica rice cultivar 'Tadukan' against blast fungal isolate B 157, using molecular tools. F-2 segregating population was derived from 'CO39' (susceptible) and 'Tadukan' (resistant), and molecular mapping of the blast resistance gene was carried out using simple sequence length polymorphism (SSLP) and amplified fragment length polymorphism (AFLP) methods. Two SSLP markers, RM206 and RM21 and three AFLP markers (AF1: E-aca/M-ctt; AF2: E-aca/M-cat and AF3: E-ace/M-cac2) were identified to be linked to the resistance gene. The co-segregation analysis using SSLP markers implied that the blast resistance gene designated Pi38 resides on rice chromosome 11.
引用
收藏
页码：596 / 599
页数：4
相关论文
共 27 条
  • [1] Bonman J. M., 1988, Oryza, V25, P103
  • [2] A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta
    Bryan, GT
    Wu, KS
    Farrall, L
    Jia, YL
    Hershey, HP
    McAdams, SA
    Faulk, KN
    Donaldson, GK
    Tarchini, R
    Valent, B
    [J]. PLANT CELL, 2000, 12 (11) : 2033 - 2045
  • [3] Development of co-dominant amplified polymorphic sequence markers in rice that flank the Magnaporthe grisea resistance gene Pi7(t) in recombinant inbred line 29
    Campbell, MA
    Chen, D
    Ronald, PC
    [J]. PHYTOPATHOLOGY, 2004, 94 (03) : 302 - 307
  • [4] Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.)
    Chen, X
    Temnykh, S
    Xu, Y
    Cho, YG
    McCouch, SR
    [J]. THEORETICAL AND APPLIED GENETICS, 1997, 95 (04) : 553 - 567
  • [5] Cloning and mapping of variety-specific rice genomic DNA sequences: Amplified fragment length polymorphisms (AFLP) from silver-stained polyacrylamide gels
    Cho, YG
    Blair, MW
    Panaud, O
    McCouch, SR
    [J]. GENOME, 1996, 39 (02) : 373 - 378
  • [6] Genome analysis of the plant pathogenic ascomycete Leptosphaeria maculans; mapping mating type and host specificity loci
    Cozijnsen, Anton J.
    Popa, Kerryn M.
    Purwantara, Agus
    Rolls, Barbara D.
    Howlett, Barbara J.
    [J]. MOLECULAR PLANT PATHOLOGY, 2000, 1 (05) : 293 - 302
  • [7] The genome sequence of the rice blast fungus Magnaporthe grisea
    Dean, RA
    Talbot, NJ
    Ebbole, DJ
    Farman, ML
    Mitchell, TK
    Orbach, MJ
    Thon, M
    Kulkarni, R
    Xu, JR
    Pan, HQ
    Read, ND
    Lee, YH
    Carbone, I
    Brown, D
    Oh, YY
    Donofrio, N
    Jeong, JS
    Soanes, DM
    Djonovic, S
    Kolomiets, E
    Rehmeyer, C
    Li, WX
    Harding, M
    Kim, S
    Lebrun, MH
    Bohnert, H
    Coughlan, S
    Butler, J
    Calvo, S
    Ma, LJ
    Nicol, R
    Purcell, S
    Nusbaum, C
    Galagan, JE
    Birren, BW
    [J]. NATURE, 2005, 434 (7036) : 980 - 986
  • [8] *IRRI, 1995, PAR IRRI CROSS
  • [9] MG-SINE - A SHORT INTERSPERSED NUCLEAR-ELEMENT FROM THE RICE BLAST FUNGUS, MAGNAPORTHE-GRISEA
    KACHROO, P
    LEONG, SA
    CHATTOO, BB
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) : 11125 - 11129
  • [10] Kinoshita T., 1998, RICE GENET NEWSL, V15, P13
123