Validation of a HPLC-UV method for the quantification of budesonide in skin layers

被引：39

作者：

Demurtas, Anna ^{[1
]}

Pescina, Silvia ^{[1
]}

Nicoli, Sara ^{[1
]}

Santi, Patrizia ^{[1
]}

de Araujo, Daniele Ribeiro ^{[2
]}

Padula, Cristina ^{[1
]}

机构：

[1] Univ Parma, Dept Food & Drug, Parco Area Sci 27-A, I-43124 Parma, Italy

[2] Fed Univ ABC, Human & Nat Sci Ctr, Ave Estados 5001,Bloco A,Torre 3, BR-09210580 Santo Andre, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2021年 / 1164卷

关键词：

Budesonide; Skin layers; HPLC-UV; Topical formulation; Skin diseases; DOUBLE-BLIND TRIAL; LIQUID; EFFICACY;

D O I：

10.1016/j.jchromb.2020.122512

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive HPLC method for the quantification of budesonide in skin layers was developed and validated. Budesonide was extracted from stratum corneum, epidermis and dermis by means of a mixture of acetonitrile:water (recovery > 90%). Budesonide quantification was performed with a RP-C18 column using methanol and water mixture (69:31, v/v) as mobile phase, pumped at 0.8 ml/min. The absorbance was monitored at 254 nm. The method resulted to be selective, linear in the range 0.05-5 or 10 mu g/ml, precise and accurate. LLOQ resulted to be 0.05 mu g/ml. The developed method appeared to be appropriate for the quantification of budesonide in skin layers at the end of in vitro permeation experiments since the recovery of the applied dose was 97 +/- 1%, in line with requirement of the OECD guideline for the testing of the chemicals (Skin absorption: in vitro method).

引用

页数：5