The purB gene of Escherichia coli K-12 is located in an operon

被引：20

作者：

Green, SM ^{[1
]}

Malik, T ^{[1
]}

Giles, IG ^{[1
]}

Drabble, WT ^{[1
]}

机构：

[1] UNIV SOUTHAMPTON,DEPT BIOCHEM,SOUTHAMPTON SO16 7PX,HANTS,ENGLAND

来源：

MICROBIOLOGY-UK | 1996年 / 142卷

关键词：

Escherichia coli; purB gene; succinyl-AMP lyase; internal promoter; phoP gene;

D O I：

10.1099/13500872-142-11-3219

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The structural gene (purB) for succinyl-AMP (S-AMP) lyase and three additional ORFs are on the same DNA strand of the chromosome of Escherichia coli. Cassette mutagenesis and primer extension mapping demonstrated that purB is co-transcribed with an upstream gene (ORF23. or ycfC) encoding a 22.9 kDa membrane-associated protein of non-essential. but unknown, function unrelated to purine biosynthesis. The purB operon lies between phoP and an ORF expressing an essential function which may correspond to asuE (trmU). S-AMP lyase was purified to near homogeneity. The purified enzyme is a homotetramer of 50 kDa subunits, has a K-m for S-AMP of 3.7 mu M and a pH optimum of 7.4-7.6.

引用

页码：3219 / 3230

页数：12

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