Histone acetylation recruits the SWR1 complex to regulate active DNA demethylation in Arabidopsis

被引：86

作者：

Nie, Wen-Feng ^{[1
,2
,3
]}

Lei, Mingguang ^{[1
,2
]}

Zhang, Mingxuan ^{[1
,4
]}

Tang, Kai ^{[1
,2
]}

Huang, Huan ^{[1
]}

Zhang, Cuijun ^{[1
,2
]}

Miki, Daisuke ^{[1
]}

Liu, Pan ^{[1
]}

Yang, Yu ^{[1
]}

Wang, Xingang ^{[2
]}

Zhang, Heng ^{[1
]}

Lang, Zhaobo ^{[1
]}

Liu, Na ^{[5
]}

Xu, Xuechen ^{[3
]}

Yelagandula, Ramesh ^{[6
]}

Zhang, Huiming ^{[1
]}

Wang, Zhidan ^{[1
]}

Chai, Xiaoqiang ^{[1
]}

Andreucci, Andrea ^{[7
]}

Yu, Jing-Quan ^{[3
]}

Berger, Frederic ^{[6
]}

Lozano-Duran, Rosa ^{[1
]}

Zhu, Jian-Kang ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Shanghai Ctr Plant Stress Biol, Shanghai 201602, Peoples R China

[2] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47906 USA

[3] Zhejiang Univ, Dept Hort, Hangzhou 310058, Zhejiang, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[5] Zhejiang Acad Agr Sci, Inst Vegetable Sci, Hangzhou 310021, Zhejiang, Peoples R China

[6] Austrian Acad Sci, Gregor Mendel Inst, A-1030 Vienna, Austria

[7] Univ Pisa, Dept Biol, I-56126 Pisa, Italy

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2019年 / 116卷 / 33期

关键词：

gene silencing; histone variant; chromatin remodeling; DNA demethylation pathway; bromodomain; LOCUS-C EXPRESSION; DOMAIN PROTEIN; VARIANT H2A.Z; METHYLATION PATTERNS; READ ALIGNMENT; FLOWERING TIME; CHROMATIN; GENE; REPRESSION; SEQUENCE;

D O I：

10.1073/pnas.1906023116

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Active DNA demethylation is critical for controlling the DNA methylomes in plants and mammals. However, little is known about how DNA demethylases are recruited to target loci, and the involvement of chromatin marks in this process. Here, we identify 2 components of the SWR1 chromatin-remodeling complex, PIE1 and ARP6, as required for ROS1-mediated DNA demethylation, and discover 2 SWR1-associated bromodomain-containing proteins, AtMBD9 and nuclear protein X1 (NPX1). AtMBD9 and NPX1 recognize histone acetylation marks established by increased DNA methylation 1 (IDM1), a known regulator of DNA demethylation, redundantly facilitating H2A.Z deposition at IDM1 target loci. We show that at some genomic regions, H2A.Z and DNA methylation marks coexist, and H2A.Z physically interacts with ROS1 to regulate DNA demethylation and antisilencing. Our results unveil a mechanism through which DNA demethylases can be recruited to specific target loci exhibiting particular histone marks, providing a conceptual framework to understand how chromatin marks regulate DNA demethylation.

引用

页码：16641 / 16650

页数：10

共 70 条

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