Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence

被引：2

作者：

Malmberg, Maja ^{[1
,2
,3
]}

Rubio-Guerri, Consuelo ^{[4
,5
]}

Hayer, Juliette ^{[2
]}

Garcia-Parraga, Daniel ^{[5
,6
]}

Nieto-Pelegrin, Elvira ^{[4
]}

Melero, Mar ^{[4
]}

Alvaro, Teresa ^{[6
]}

Valls, Monica ^{[6
]}

Manuel Sanchez-Vizcaino, Jose ^{[4
]}

Belak, Sandor ^{[1
,3
]}

Granberg, Fredrik ^{[1
,3
]}

机构：

[1] Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, Sect Virol, Box 7028, S-75007 Uppsala, Sweden

[2] Swedish Univ Agr Sci, Dept Anim Breeding & Genet, SLU Global Bioinformat Ctr, Box 7023, S-75007 Uppsala, Sweden

[3] World Org Anim Hlth OIE, Collaborating Ctr Biotechnol Based Diag Infect Di, Box 7028, S-75007 Uppsala, Sweden

[4] Univ Complutense Madrid, Vet Sch, VISAVET Ctr & Anim Hlth Dept, Av Puerta Hierro S-N, Madrid 28040, Spain

[5] Fdn Oceanog Ciudad Arte & Ciencias, Primo Yufera 1B, Valencia 46013, Spain

[6] Vet Serv, Avanqua Oceanog Agora, C Eduardo Primo Yufera 1B, Valencia 46013, Spain

来源：

INFECTION GENETICS AND EVOLUTION | 2017年 / 53卷

基金：

瑞典研究理事会;

关键词：

Adenovirus Complete genome; E3; region; Bottlenose dolphin; Tursiops truncatus; High-throughput sequencing; Viral metagenomics; VIRUS-ASSOCIATED RNA; VIRAL-HEPATITIS; READ ALIGNMENT; EVOLUTION; INFECTION; INFERENCE; DISEASES; DISTINCT; VECTORS; DUTPASE;

D O I：

10.1016/j.meegid.2017.05.008

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Adenoviruses are common pathogens in vertebrates, infecting a wide range of hosts, but only having rarely been detected and correlated with disease in cetaceans. This article describes the first complete genomic sequence of a cetacean adenovirus, bottlenose dolphin adenovirus 1 (BdAdV-1), detected in captive bottlenose dolphin population ( Tursiops truncatus) suffering from self-limiting gastroenteritis. The complete genome sequence of BdAdV-1 was recovered from data generated by high-throughput sequencing and validated by Sanger sequencing. The genome is 34,080 bp long and has 220 nucleotides long inverted terminal repeats. A total of 29 coding sequences were identified, 26 of which were functionally annotated. Among the unusual features of this genome is a remarkably long 4380 bp E3 ORF1, that displays no sequence homology with the corresponding E3 regions of other adenoviruses. In addition, the fiber protein only has 26% identity with fiber proteins described in other adenoviruses. Three hypothetical proteins were predicted. The phylogenetic analysis indicates that the closest known relative to BdAdV-1 is an adenovirus detected in bottlenose dolphin (KR024710), with an amino acid sequence identity between 36 and 79% depending on the protein. Based on the phylogenic analysis, the BdAdV-1 appears to have co-evolved with its host. The results indicate that BdAdV-1 belongs to the Mastadenovirus genus of the Adenoviridae family, however, it is clearly different from other adenoviruses, especially in the 3'-end of the viral genome. The high degree of sequence divergence suggests that BdAdV-1 should be considered as a novel species in the Mastadenovirus genus. The study also demonstrates the usefulness of high-throughput sequencing to obtain full-length genomes of genetically divergent viruses. (C) 2017 The Authors. Published by Elsevier B.V.

引用

页码：47 / 55

页数：9