Divergent and convergent modes of interaction between wheat and Puccinia graminis f. sp tritici isolates revealed by the comparative gene co-expression network and genome analyses

被引:19
作者
Rutter, William B. [1 ,4 ]
Salcedo, Andres [1 ]
Akhunova, Alina [2 ]
He, Fei [1 ]
Wang, Shichen [1 ,5 ]
Liang, Hanquan [2 ,6 ]
Bowden, Robert L. [3 ]
Akhunov, Eduard [1 ]
机构
[1] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA
[2] Kansas State Univ, Integrated Genom Facil, Manhattan, KS 66506 USA
[3] USDA ARS, Hard Winter Wheat Genet Res Unit, Throckmorton Plant Sci Ctr, Manhattan, KS 66506 USA
[4] USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA
[5] Texas A&M Univ, TEES AgriLife Ctr Bioinformat & Genom Syst Engn, 101 Gateway,Suite A, College Stn, TX 77845 USA
[6] Sun Yat Sen Univ, Sch Data & Comp Sci, Guangzhou 510006, Guangdong, Peoples R China
来源
BMC GENOMICS | 2017年 / 18卷
关键词
Wheat resistance; Stem rust; Comparative genomics; Gene co-expression network; STEM RUST; EXPRESSION ANALYSIS; EFFECTOR PROTEINS; RESISTANCE; VIRULENCE; DEFENSE; IDENTIFICATION; PATHOGENS; ARABIDOPSIS; PROSPECTS;
D O I
10.1186/s12864-017-3678-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Two opposing evolutionary constraints exert pressure on plant pathogens: one to diversify virulence factors in order to evade plant defenses, and the other to retain virulence factors critical for maintaining a compatible interaction with the plant host. To better understand how the diversified arsenals of fungal genes promote interaction with the same compatible wheat line, we performed a comparative genomic analysis of two North American isolates of Puccinia graminis f. sp. tritici (Pgt). Results: The patterns of inter-isolate divergence in the secreted candidate effector genes were compared with the levels of conservation and divergence of plant-pathogen gene co-expression networks (GCN) developed for each isolate. Comprative genomic analyses revealed substantial level of interisolate divergence in effector gene complement and sequence divergence. Gene Ontology (GO) analyses of the conserved and unique parts of the isolate-specific GCNs identified a number of conserved host pathways targeted by both isolates. Interestingly, the degree of inter-isolate sub-network conservation varied widely for the different host pathways and was positively associated with the proportion of conserved effector candidates associated with each sub- network. While different Pgt isolates tended to exploit similar wheat pathways for infection, the mode of plant-pathogen interaction varied for different pathways with some pathways being associated with the conserved set of effectors and others being linked with the diverged or isolate-specific effectors. Conclusions: Our data suggest that at the intra-species level pathogen populations likely maintain divergent sets of effectors capable of targeting the same plant host pathways. This functional redundancy may play an important role in the dynamic of the "arms-race" between host and pathogen serving as the basis for diverse virulence strategies and creating conditions where mutations in certain effector groups will not have a major effect on the pathogen's ability to infect the host.
引用
收藏
页数:20
相关论文
共 73 条
[1]   Discovery of Core Biotic Stress Responsive Genes in Arabidopsis by Weighted Gene Co-Expression Network Analysis [J].
Amrine, Katherine C. H. ;
Blanco-Ulate, Barbara ;
Cantu, Dario .
PLOS ONE, 2015, 10 (03)
[2]   Plants versus pathogens: an evolutionary arms race [J].
Anderson, Jonathan P. ;
Gleason, Cynthia A. ;
Foley, Rhonda C. ;
Thrall, Peter H. ;
Burdon, Jeremy B. ;
Singh, Karam B. .
FUNCTIONAL PLANT BIOLOGY, 2010, 37 (06) :499-512
[3]  
[Anonymous], SCIENCE
[4]  
[Anonymous], 2016, FRONT MICROBIOL
[5]  
[Anonymous], BMC GENOMICS
[6]  
[Anonymous], INT J PLANT GENOMICS
[7]  
[Anonymous], FRONT PLANT SCI
[8]   Trafficking arms: oomycete effectors enter host plant cells [J].
Birch, PRJ ;
Rehmany, AP ;
Pritchard, L ;
Kamoun, S ;
Beynon, JL .
TRENDS IN MICROBIOLOGY, 2006, 14 (01) :8-11
[9]   Multiple Avirulence Loci and Allele-Specific Effector Recognition Control the Pm3 Race-Specific Resistance of Wheat to Powdery Mildew [J].
Bourras, Salim ;
McNally, Kaitlin Elyse ;
Ben-David, Roi ;
Parlange, Francis ;
Roffler, Stefan ;
Praz, Coraline Rosalie ;
Oberhaensli, Simone ;
Menardo, Fabrizio ;
Stirnweis, Daniel ;
Frenkel, Zeev ;
Schaefer, Luisa Katharina ;
Flueckiger, Simon ;
Treier, Georges ;
Herren, Gerhard ;
Korol, Abraham B. ;
Wicker, Thomas ;
Keller, Beat .
PLANT CELL, 2015, 27 (10) :2991-3012
[10]   Using transcription of six Puccinia triticina races to identify the effective secretome during infection of wheat [J].
Bruce, Myron ;
Neugebauer, Kerri A. ;
Joly, David L. ;
Migeon, Pierre ;
Cuomo, Christina A. ;
Wang, Shichen ;
Akhunov, Eduard ;
Bakkeren, Guus ;
Kolmer, James A. ;
Fellers, John P. .
FRONTIERS IN PLANT SCIENCE, 2014, 4