A transgenic RNAi approach for developing tomato plants immune to Pepino mosaic virus

被引：6

作者：

Leibman, Diana ^{[1
]}

Ortega-Parra, Nelia ^{[2
,3
]}

Wolf, Dalia ^{[1
]}

Shterkman, Meital ^{[1
]}

Hanssen, Inge ^{[2
,4
]}

Gal-On, Amit ^{[1
]}

机构：

[1] ARO Volcani Ctr, Dept Plant Pathol & Weed Sci, POB 15159, IL-7528809 Rishon Leziyyon, Israel

[2] Scientia Terrae Res Inst STRI, St Katelijne Waver, Belgium

[3] De Ceuster Meststoffen BV DCM, Katwijk Aan Zee, Netherlands

[4] De Ceuster Meststoffen NV DCM, Grobbendonk, Belgium

来源：

PLANT PATHOLOGY | 2021年 / 70卷 / 04期

关键词：

immunity; Pepino mosaic virus; tomato; transgenic;

D O I：

10.1111/ppa.13346

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

RNA interference (RNAi) or gene silencing is a natural defence response of plants to invading viruses. Here, we applied this approach against pepino mosaic virus (PepMV) isolates in their natural host, tomato. PepMV isolates differ in their genetic sequences, the severity of the disease they induce, and their worldwide distribution. PepMV causes heavy crop losses, mainly due to impaired tomato fruit quality. Resistant varieties are not yet available, despite many years of resistance breeding efforts within the tomato seed industry. To generate broad resistance to PepMV strains, conserved sequences from three different strains of PepMV (US1, LP, and CH2) were synthesized as a single insert and cloned in a hairpin configuration into a binary vector, which was used to transform tomato plants. Transgenic tomato lines that expressed a high level of transgene-siRNA exhibited immunity to PepMV strains, including a new Israeli isolate. This immunity was maintained even after graft inoculation, in which a transgenic scion was grafted onto nontransgenic infected rootstocks. However, an immune transgenic rootstock was unable to induce resistance in a nontransformed scion. These results provide the first example of engineered immunity to diverse PepMV strains in transgenic tomato based on gene silencing.

引用

页码：1003 / 1012

页数：10