Endogenous cellulase enzymes in the stick insect (Phasmatodea) gut

被引：32

作者：

Shelomi, Matan ^{[1
]}

Watanabe, Hirofumi ^{[2
]}

Arakawa, Gaku ^{[2
]}

机构：

[1] Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA

[2] Natl Inst Agrobiol Sci, Insect Mimet Res Unit, Tsukuba, Ibaraki 3058634, Japan

来源：

JOURNAL OF INSECT PHYSIOLOGY | 2014年 / 60卷

基金：

日本学术振兴会; 美国国家科学基金会;

关键词：

Phasmatodea; Cellulase; Endoglucanase; Digestion; Eurycantha calcarata; Entoria okinawaensis; ORTHOPTERA; PHYLOGENY; DIGESTION; PROTEINS; SYSTEMS;

D O I：

10.1016/j.jinsphys.2013.10.007

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

High cellulase (endo-beta-1,4-glucanase) activity was detected in the anterior midgut of the walking stick (Phasmatodea) Eurycantha calcarata. The enzyme was isolated and analyzed via mass spectrometry. RT-PCR revealed two endoglucanase genes, EcEG1 and EcEG2. Mascot analysis of the purified enzyme confirms it to be the product of gene EcEG1. Homologous cDNAs were also isolated from a distantly related species, Entoria okinawaensis, suggesting a general distribution of cellulase genes in phasmids. Phasmid cellulases showed high homology to endogenously-produced glycoside hydrolase family 9 (GH9) endoglucanases from insects, especially to those of termites, cockroaches, and crickets. The purified E. calcarata enzyme showed clear antigency against an anti-serum for termite GH9 cellulase, which, together with the sequence homology, further suggests an endogenous origin of the enzyme. This discovery suggests a possible nutritive value for cellulose in the leaf-feeding phasmids, unlike in herbivorous Lepidoptera. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.

引用

页码：25 / 30

页数：6

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