Nine new polymorphic microsatellite loci for the amplification of archived otolith DNA of Atlantic cod, Gadus morhua L.

被引：34

作者：

Jakobsdottir, Klara Bjorg

Jorundsdottir, Ora Dogg

Skirnisdottir, Sigurlaug

Hjorleifsdottir, Sigridur

Hreggvidsson, Gudmundur O.

Danielsdottir, Anna Kristin

Pampoulie, Christophe

机构：

[1] Marine Res Inst, IS-121 Reykjavik, Iceland

[2] Univ Iceland, Dept Biol, IS-101 Reykjavik, Iceland

[3] Prokaria Ltd, IS-112 Reykjavik, Iceland

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 02期

关键词：

archived DNA; Atlantic cod; Gadus morhua; microsatellite;

D O I：

10.1111/j.1471-8286.2005.01223.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine out of 22 microsatellite primers tested were successfully amplified on three samples of cod Gadus morhua L. (two contemporary and one archived otolith samples). All loci were polymorphic (5-23 alleles/locus). The average observed heterozygosity across loci and samples was 0.625, ranging from 0.294 to 0.895 at each locus. All loci were under Hardy-Weinberg equilibrium, except PGmo56 that showed significant excess of heterozygotes in all studied samples. The isolated loci were suitable for degraded DNA and therefore useful for conducting a long-term temporal study with DNA obtained from archived otoliths of cod.

引用

页码：337 / 339

页数：3

共 12 条

[1] Hundred-year decline of North Atlantic predatory fishes [J].