Dysfunction of cortical synapse-specific mitochondria in developing rats exposed to lead and its amelioration by ascorbate supplementation

被引:13
作者
Ahmad, Faraz [1 ,2 ]
Salahuddin, Mohammad [3 ]
Alamoudi, Widyan [2 ]
Acharya, Sadananda [1 ]
机构
[1] Imam Abdulrahman Bin Faisal Univ, Coll Publ Hlth, Dept Publ Hlth, Dammam 31441, Saudi Arabia
[2] Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat, Neurosci Dept, Dammam, Saudi Arabia
[3] Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat, Anim House Dept, Dammam, Saudi Arabia
关键词
synaptic; oxidative damage; heavy metal neurotoxicity; neuropsychiatric; mitochondrial bioenergetics; mitochondrial membrane potential; INDUCED OXIDATIVE STRESS; PERMEABILITY TRANSITION; PERINATAL EXPOSURE; VITAMIN-C; BRAIN; ACID; NEUROTOXICITY; CELLS; NEURODEGENERATION; HIPPOCAMPUS;
D O I
10.2147/NDT.S148248
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Background: Lead (Pb) is a widespread environmental neurotoxin and its exposure even in minute quantities can lead to compromised neuronal functions. A developing brain is particularly vulnerable to Pb mediated toxicity and early-life exposure leads to permanent alterations in brain development and neuronal signaling and plasticity, culminating into cognitive and behavioral dysfunctions and elevated risk of neuropsychiatric disorders later in life. Nevertheless, the underlying biochemical mechanisms have not been completely discerned. Methods: Because of their ability to fulfill high energy needs and to act as calcium buffers in events of high intensity neuronal activity as well as their adaptive regulatory capability to match the requirements of the dynamicity of synaptic signaling, synapse-specific or synaptic mitochondria (SM) are critical for synaptic development, function and plasticity. Our aim for the present study hence was to characterize the effects of early-life Pb exposure on the functions of SM of prepubertal rats. For this purpose, employing a chronic model of Pb neurotoxicity, we exposed rat pups perinatally and postnatally to Pb and used a plethora of colorimetric and fluorometric assays for assessing redox and bioenergetic properties of SM. In addition, taking advantage of its ability as an antioxidant and as a metal chelator, we employed ascorbic acid (vitamin C) supplementation as an ameliorative therapeutic strategy against Pb-induced neurotoxicity and dysfunction of SM. Results: Our results suggest that early-life exposure to Pb leads to elevated oxidative stress in cortical SM with consequent compromises in its energy metabolism activity. Ascorbate supplementation resulted in significant recovery of Pb-induced oxidative stress and functional compromise of SM. Conclusion: Alterations in redox status and bioenergetic properties of SM could potentially contribute to the synaptic dysfunction observed in events of Pb neurotoxicity. Additionally, our study provides evidence for suitability of ascorbate as a significant ameliorative agent in tacking Pb neurotoxicity.
引用
收藏
页码:813 / 824
页数:12
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