Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum

被引：36

作者：

Hentschel, Eva ^{[1
]}

Will, Cornelia ^{[2
]}

Mustafi, Nurije ^{[1
]}

Burkovski, Andreas ^{[2
]}

Rehm, Nadine ^{[2
]}

Frunzke, Julia ^{[1
]}

机构：

[1] Forschungszentrum Julich, Inst Bio & Geowissensch, IBG Biotechnol 1, D-52425 Julich, Germany

[2] Univ Erlangen Nurnberg, Lehrstuhl Mikrobiol, D-91054 Erlangen, Germany

来源：

MICROBIAL BIOTECHNOLOGY | 2013年 / 6卷 / 02期

关键词：

GREEN FLUORESCENT PROTEIN; REPORTER PROTEINS; SEQUENCE; VECTORS;

D O I：

10.1111/j.1751-7915.2012.00360.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum.

引用

页码：196 / 201

页数：6

共 27 条

[1]

Allen Michael S, 2007, Syst Synth Biol, V1, P3, DOI 10.1007/s11693-006-9001

[2]

Andersen JB, 1998, APPL ENVIRON MICROB, V64, P2240

[3] Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression [J].

Blokpoel, MCJ ;

O'Toole, R ;

Smeulders, MJ ;

Williams, HD .

JOURNAL OF MICROBIOLOGICAL METHODS, 2003, 54 (02) :203-211

[4] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[5] CONTROL OF THE LYSINE BIOSYNTHESIS SEQUENCE IN CORYNEBACTERIUM-GLUTAMICUM AS ANALYZED BY OVEREXPRESSION OF THE INDIVIDUAL CORRESPONDING GENES [J].

CREMER, J ;

EGGELING, L ;

SAHM, H .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (06) :1746-1752

[6] Construction of an l-phenylalanine-producing tyrosine-prototrophic Escherichia coli strain using tyrA ssrA-like tagged alleles [J].

Doroshenko, Vera G. ;

Shakulov, Rustem S. ;

Kazakova, Svetlana M. ;

Kivero, Alexander D. ;

Yampolskaya, Tatyana A. ;

Mashko, Sergey V. .

BIOTECHNOLOGY LETTERS, 2010, 32 (08) :1117-1121

[7] Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters [J].

Drepper, Thomas ;

Huber, Robert ;

Heck, Achim ;

Circolone, Franco ;

Hillmer, Anne-Kathrin ;

Buechs, Jochen ;

Jaeger, Karl-Erich .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (17) :5990-5994

[8]

Eggeling L., 2005, Handbook of Corynebacterium glutamicum

[9] A FAMILY OF CORYNEBACTERIUM-GLUTAMICUM ESCHERICHIA-COLI SHUTTLE VECTORS FOR CLONING, CONTROLLED GENE-EXPRESSION, AND PROMOTER PROBING [J].

EIKMANNS, BJ ;

KLEINERTZ, E ;

LIEBL, W ;

SAHM, H .

GENE, 1991, 102 (01) :93-98

[10] A synthetic oscillatory network of transcriptional regulators [J].

Elowitz, MB ;

Leibler, S .

NATURE, 2000, 403 (6767) :335-338

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