Molecular epidemiology of betanodavirus-Sequence analysis strategies and quasispecies influence outbreak source attribution

被引：6

作者：

Hick, Paul ^{[1
]}

Gore, Kylie ^{[1
]}

Whittington, Richard ^{[1
]}

机构：

[1] Univ Sydney, Fac Vet Sci, Camden, NSW 2570, Australia

来源：

VIROLOGY | 2013年 / 436卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

Betanodavirus; Horizontal transmission; Molecular epidemiology; Quasispecies; Viral nervous necrosis; SALMON-ANEMIA-VIRUS; PHYLOGENETIC ANALYSIS; NERVOUS NECROSIS; GENOMIC CLASSIFICATION; FISH NODAVIRUSES; DISEASE VIRUS; RNA VIRUS; PROTEIN; TRANSMISSION; OPTIMIZATION;

D O I：

10.1016/j.virol.2012.10.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The quasispecies structure of nervous necrosis virus (NNV) was determined to investigate an outbreak of viral nervous necrosis disease at a barramundi (Lates calcarifer) hatchery. A traditional epidemiological investigation indicated horizontal transmission of infection between two cohorts of fish. However, variation in the viral capsid protein gene sequence from cell culture-derived viral populations and from individual fish suggested that each cohort was infected with a different virus. Molecular support for the correct epidemiological conclusion was provided by determining the consensus NNV sequence directly from multiple fish, to show that each cohort was infected with the same quasispecies. Variation in the capsid gene of isolates obtained from this quasispecies was up to 3.3% compared with sequences determined directly from fish tissue, and <= 1.7% between individual fish within each cohort. Determination of the NNV quasispecies structure supported implementation of biosecurity measures to protect fish in the hatchery from environmental sources of infection. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：15 / 23

页数：9

共 44 条

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