The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101

被引：500
作者：
Stephens, LR
Eguinoa, A
ErdjumentBromage, H
Lui, M
Cooke, F
Coadwell, J
Smrcka, AS
Thelen, M
Cadwallader, K
Tempst, P
Hawkins, PT
机构：
[1] MEM SLOAN KETTERING CANC CTR, PROGRAM MOL BIOL, NEW YORK, NY 10021 USA
[2] UNIV ROCHESTER, DEPT PHYSIOL & PHARMACOL, ROCHESTER, NY 14642 USA
[3] UNIV BERN, THEODOR KOCHER INST, CH-3000 BERN 9, SWITZERLAND
[4] ONYX PHARMACEUT, RICHMOND, CA 94806 USA
来源：
CELL | 1997年 / 89卷 / 01期
关键词：
D O I：
10.1016/S0092-8674(00)80187-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two highly similar, PtdIns(4,5)P-2-selective, G beta gamma-activated PI3Ks were purified from pig neutrophil cytosol. Both were heterodimers, were composed of a 101 kDa protein and either a 120 kDa or a 117 kDa catalytic subunit, and were activated greater than 100-fold by G beta gamma s. Peptide sequence-based oligonucleotide probes were used to clone cDNAs for the p120 and p101 species. The cDNA of p120 is highly related to p110 gamma, while the cDNA of p101 is not substantially related to anything in current databases. The proteins were expressed in and purified from insect and mammalian cells. They bound tightly to one another, both in vivo and in vitro, and in so doing, p101 amplified the effect of G beta gamma s on the PI3K activity of p120 from less than 2-fold to greater than 100-fold.
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页码：105 / 114
页数：10
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