Phosphonate analogs of 2-oxoglutarate perturb metabolism and gene expression in illuminated Arabidopsis leaves

被引:28
|
作者
Araujo, Wagner L. [1 ,2 ]
Tohge, Takayuki [1 ]
Nunes-Nesi, Adriano [3 ]
Daloso, Danilo M. [1 ,2 ]
Nimick, Mhairi [4 ]
Krahnert, Ina [1 ]
Bunik, Victoria I. [5 ]
Moorhead, Greg B. G. [4 ]
Fernie, Alisdair R. [1 ]
机构
[1] Max Planck Inst Mol Pflanzenphysiol, D-14476 Potsdam, Germany
[2] Univ Fed Vicosa, Dept Biol Vegetal, Vicosa, MG, Brazil
[3] Univ Fed Vicosa, Max Planck Partner Grp, Dept Biol Vegetal, Vicosa, MG, Brazil
[4] Univ Calgary, Dept Biol Sci, Calgary, AB T2N 1N4, Canada
[5] Moscow MV Lomonosov State Univ, AN Belozersly Inst Physicochem Biol, Moscow, Russia
来源
FRONTIERS IN PLANT SCIENCE | 2012年 / 3卷
基金
加拿大自然科学与工程研究理事会;
关键词
2-oxoglutarate dehydrogenase; phosphonate inhibitors; TCA cycle; PII; 2-oxoglutarate; GABA;
D O I
10.3389/fpls.2012.00114
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Although the role of the 2-oxoglutarate dehydrogenase complex (2-OGDHC) has previously been demonstrated in plant heterotrophic tissues its role in photosynthetically active tissues remains poorly understood. By using a combination of metabolite and transcript profiles we here investigated the function of 2-OGDHC in leaves of Arabidopsis thaliana via use of specific phosphonate inhibitors of the enzyme. Incubation of leaf disks with the inhibitors revealed that they produced the anticipated effects on the in situ enzyme activity. In vitro experiments revealed that succinyl phosphonate (SP) and a carboxy ethyl ester of SP are slow-binding inhibitors of the 2-OGDHC. Our results indicate that the reduced respiration rates are associated with changes in the regulation of metabolic and signaling pathways leading to an imbalance in carbon-nitrogen metabolism and cell homeostasis. The inducible alteration of primary metabolism was associated with altered expression of genes belonging to networks of amino acids, plant respiration, and sugar metabolism. In addition, by using isothermal titration calorimetry we excluded the possibility that the changes in gene expression resulted from an effect on 2-oxoglutarate (2OG) binding to the carbon/ATP sensing protein PII. We also demonstrated that the 2OG degradation by the 2-oxoglutarate dehydrogenase strongly influences the distribution of intermediates of the tricarboxylic acid (TCA) cycle and the GABA shunt. Our results indicate that the TCA cycle activity is clearly working in a non-cyclic manner upon 2-OGDHC inhibition during the light period
引用
收藏
页数:19
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