Pannexin 1 forms an anion-selective channel

被引：102

作者：

Ma, Weihong ^{[1
]}

Compan, Vincent ^{[1
]}

Zheng, Wenxuan ^{[1
]}

Martin, Elizabeth ^{[1
]}

North, R. Alan ^{[1
]}

Verkhratsky, Alexei ^{[1
]}

Surprenant, Annmarie ^{[1
]}

机构：

[1] Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 2012年 / 463卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Pannexin; 1; HEK293; Anion channel; Single channel; GAP-JUNCTION PROTEINS; MEMBRANE-PERMEABILITY; CELL COMMUNICATION; P2X(7) RECEPTOR; TASTE-BUDS; HEMICHANNELS; ATP; RELEASE; NEURONS; FAMILY;

D O I：

10.1007/s00424-012-1077-z

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Pannexin 1 (Panx1) is expressed in various mammalian tissues including the brain and immune cells. Here, we present evidence that Panx1 when expressed in mammalian cells, forms anion-selective channels, with a rank order of permeabilities: NO (3) (-) > I- > Br- > Cl- > F- a parts per thousand << aEuro parts per thousand aspartate(-) a parts per thousand aEuro parts per thousand glutamate(-) a parts per thousand aEuro parts per thousand gluconate(-). Single-channel Panx1-mediated currents have a unitary conductance around 68 pS. Our results show that Panx1 assembles into a membrane anion channel with a relatively low single-channel conductance.

引用

页码：585 / 592

页数：8

共 30 条

[1] Pannexin membrane channels are mechanosensitive conduits for ATP [J].

Bao, L ;

Locovei, S ;

Dahl, G .

FEBS LETTERS, 2004, 572 (1-3) :65-68

[2] The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins [J].

Baranova, A ;

Ivanova, DV ;

Petrash, N ;

Pestova, A ;

Skoblov, M ;

Kelmanson, I ;

Shagin, D ;

Nazarenko, S ;

Geraymovych, E ;

Litvin, O ;

Tiunova, A ;

Born, TL ;

Usman, N ;

Staroverov, D ;

Lukyanov, S ;

Panchin, Y .

GENOMICS, 2004, 83 (04) :706-716

[3] Pannexin1 channels contain a glycosylation site that targets the hexamer to the plasma membrane [J].

Boassa, Daniela ;

Ambrosi, Cinzia ;

Qiu, Feng ;

Dahl, Gerhard ;

Gaietta, Guido ;

Sosinsky, Gina .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (43) :31733-31743

[4] Pharmacological properties of homomeric and heteromeric pannexin hemichannels expressed in Xenopus oocytes [J].

Bruzzone, R ;

Barbe, MT ;

Jakob, NJ ;

Monyer, H .

JOURNAL OF NEUROCHEMISTRY, 2005, 92 (05) :1033-1043

[5] Pannexins, a family of gap junction proteins expressed in brain [J].

Bruzzone, R ;

Hormuzdi, SG ;

Barbe, MT ;

Herb, A ;

Monyer, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (23) :13644-13649

[6] Pannexin 1 channels mediate 'find-me' signal release and membrane permeability during apoptosis [J].

Chekeni, Faraaz B. ;

Elliott, Michael R. ;

Sandilos, Joanna K. ;

Walk, Scott F. ;

Kinchen, Jason M. ;

Lazarowski, Eduardo R. ;

Armstrong, Allison J. ;

Penuela, Silvia ;

Laird, Dale W. ;

Salvesen, Guy S. ;

Isakson, Brant E. ;

Bayliss, Douglas A. ;

Ravichandran, Kodi S. .

NATURE, 2010, 467 (7317) :863-U136

[7] Cell-to-cell communication in intact taste buds through ATP signalling from pannexin 1 gap junction hemichannels [J].

Dando, Robin ;

Roper, Stephen D. .

JOURNAL OF PHYSIOLOGY-LONDON, 2009, 587 (24) :5899-5906

[8] EXPRESSION OF GAP JUNCTION CHANNELS IN COMMUNICATION-INCOMPETENT CELLS AFTER STABLE TRANSFECTION WITH CDNA-ENCODING CONNEXIN-32 [J].

EGHBALI, B ;

KESSLER, JA ;

SPRAY, DC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1328-1331

[9] Nonsynaptic and nonvesicular ATP release from neurons and relevance to neuron-glia signaling [J].

Fields, R. Douglas .

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2011, 22 (02) :214-219

[10] Pannexin1 is expressed by neurons and glia but does not form functional gap junctions [J].

Huang, Yan ;

Grinspan, Judith B. ;

Abrams, Charles K. ;

Scherer, Steven S. .

GLIA, 2007, 55 (01) :46-56

← 1 2 3 →