Nanowire-integrated microfluidic devices for facile and reagent-free mechanical cell lysis

被引：66

作者：

Kim, Jung ^{[1
]}

Hong, Jung Woo ^{[2
]}

Kim, Dong Pyo ^{[3
]}

Shin, Jennifer H. ^{[2
]}

Park, Inkyu ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Div Mech Engn, Sch Mech Aerosp & Syst Engn, Multifunct & Integrated Nanosyst Technol MINT Grp, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Soft Biomech & Biomat Lab, Sch Mech Aerosp & Syst Engn, Div Mech Engn, Taejon 305701, South Korea

[3] POSTECH, Dept Chem Engn, Natl Creat Res Ctr Appl Microfluid Chem, Pohang, South Korea

来源：

LAB ON A CHIP | 2012年 / 12卷 / 16期

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1039/c2lc40154a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell lysis is an essential task for the detection of intracellular components. In this work, we introduce novel microfluidic devices integrated with patterned one-dimensional nanostructure arrays for facile and high-throughput mechanical cell lysis. The geometry of the hydrothermally grown ZnO nanowires, characterised by sharp tips and high aspect ratios, aids in anchoring the cell and tearing the plasma membrane, enabling simple and highly efficient extraction of cellular proteins and nucleic acids. This method lyses cells more effectively than conventional chemical lysis methods with simpler equipment and a shorter processing time.

引用

页码：2914 / 2921

页数：8

共 17 条

[1] Cell lysis inside the capillary facilitated by transverse diffusion of laminar flow profiles (TDLFP) [J].