Plant regeneration in Actinidia polygama Miq. by leaf, stem, and petiole culture with zeatin, and from stem-derived calli on low-sucrose medium

被引：18

作者：

Takahashi, Wataru ^{[1
]}

Sugawara, Fuyuki ^{[2
]}

Yamamoto, Noriko ^{[1
]}

Bando, Eriko ^{[1
]}

Matsushita, Junjo ^{[1
]}

Tanaka, Osamu ^{[1
]}

机构：

[1] Konan Univ, Fac Sci & Engn, Dept Biol, Kobe, Hyogo 6588501, Japan

[2] Akita Prefecture Forest Tech Ctr, Akita, Japan

来源：

JOURNAL OF FOREST RESEARCH | 2004年 / 9卷 / 01期

关键词：

Actinidia polygama Miq; Callus; Plant regeneration; Sucrose; Zeatin;

D O I：

10.1007/s10310-003-0053-z

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

We examined the effect of zeatin on the formation of shoot buds from explants and callus tissues derived from stem segments of Actinidia polygama Miq. (matatabi or silver vine). Stem and petiole segments cultured on combined broad-leaved tree medium and woody plant medium (BW medium) supplemented with zeatin for 40 days formed many shoot buds. Callus tissues derived from the stem segments and subcultured on BW medium supplemented with 6-benzyladenine and 1-naphthaleneacetic acid formed shoot buds when the medium contained 13.7 mu M zeatin. BW medium containing low concentrations of sucrose strongly induced the formation of shoot buds from the callus.

引用

页码：85 / 88

页数：4

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