Cooperative DNA Probing Using a β-Cyclodextrin-DNA Conjugate and a Nucleobase-Specific Fluorescent Ligand

被引：66

作者：

Ihara, Toshihiro ^{[1
,3
]}

Uemura, Asuka ^{[1
]}

Futamura, Akika ^{[1
]}

Shimizu, Masamichi ^{[1
]}

Baba, Noriyuki ^{[2
]}

Nishizawa, Seiichi ^{[2
,4
]}

Teramae, Norio ^{[2
,4
]}

Jyo, Akinori ^{[1
]}

机构：

[1] Kumamoto Univ, Dept Appl Chem & Biochem, Grad Sch Sci & Technol, Kumamoto 8608555, Japan

[2] Tohoku Univ, Dept Chem, Grad Sch Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

[3] Japan Sci & Technol Agcy, PRESTO, Chiyoda Ku, Kawaguchi, Saitama 3320012, Japan

[4] Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Kawaguchi, Saitama 3320012, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 04期

关键词：

ABASIC SITE; RECOGNITION; PROBES;

D O I：

10.1021/ja809023n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A single nucleotide potymorphism (SNP) base on the target is displayed at a gap in a ternary duplex carrying B-cyclodextrin-modified DNA. A stable tandem duplex forms regardless of the type of SNP base. A nucleobase-specific ligand is then added to this system. The dansyl moiety in the ligand is expected to form a luminous inclusion complex with nearby B-CyD, only when the ligand recognizes the specific base displayed in the gap.

引用

页码：1386 / +

页数：4

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