Histology-derived volumetric annotation of the human hippocampal subfields in postmortem MRI

被引:115
作者
Adler, Daniel H. [1 ,2 ]
Pluta, John [1 ,3 ]
Kadivar, Salmon [1 ]
Craige, Caryne [1 ]
Gee, James C. [1 ]
Avants, Brian B. [1 ]
Yushkevich, Paul A. [1 ]
机构
[1] Univ Penn, Dept Radiol, Penn Image Comp & Sci Lab, Philadelphia, PA 19104 USA
[2] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA
[3] Univ Penn, Dept Neurol, Ctr Funct Neuroimaging, Philadelphia, PA 19104 USA
基金
加拿大自然科学与工程研究理事会; 美国国家卫生研究院;
关键词
Hippocampus; Subfields; Histology; MRI; Reconstruction; Postmortem; IN-VIVO MRI; MILD COGNITIVE IMPAIRMENT; MEDIAL TEMPORAL-LOBE; ENTORHINAL CORTEX; MOUSE-BRAIN; 3-DIMENSIONAL REGISTRATION; IMAGE REGISTRATION; PATTERN SEPARATION; CEREBRAL-CORTEX; NEURON NUMBER;
D O I
10.1016/j.neuroimage.2013.08.067
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Recently, there has been a growing effort to analyze the morphometry of hippocampal subfields using both in vivo and postmortem magnetic resonance imaging (MRI). However, given that boundaries between subregions of the hippocampal formation (HF) are conventionally defined on the basis of microscopic features that often lack discernible signature in MRI, subfield delineation in MRI literature has largely relied on heuristic geometric rules, the validity of which with respect to the underlying anatomy is largely unknown. The development and evaluation of such rules are challenged by the limited availability of data linking MRI appearance to microscopic hippocampal anatomy, particularly in three dimensions (3D). The present paper, for the first time, demonstrates the feasibility of labeling hippocampal subfields in a high resolution volumetric MRI dataset based directly on microscopic features extracted from histology. It uses a combination of computational techniques and manual post-processing to map subfield boundaries from a stack of histology images (obtained with 200 mu m spacing and 5 mu m slice thickness; stained using the Kluver-Barrera method) onto a postmortem 9.4 Tesla MRI scan of the intact, whole hippocampal formation acquired with 160 mu m isotropic resolution. The histology reconstruction procedure consists of sequential application of a graph-theoretic slice stacking algorithm that mitigates the effects of distorted slices, followed by iterative affine and diffeomorphic co-registration to postmortem MRI scans of approximately 1 cm-thick tissue sub-blocks acquired with 200 mu m isotropic resolution. These 1 cm blocks are subsequently co-registered to the MRI of the whole HE Reconstruction accuracy is evaluated as the average displacement error between boundaries manually delineated in both the histology and MRI following the sequential stages of reconstruction. The methods presented and evaluated in this single-subject study can potentially be applied to multiple hippocampal tissue samples in order to construct a histologically informed MRI atlas of the hippocampal formation. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:505 / 523
页数:19
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