Metabolic Responses in Endothelial Cells Following Exposure to Ketone Bodies

被引:24
作者
Meroni, Erika [1 ]
Papini, Nadia [2 ]
Criscuoli, Franca [1 ]
Casiraghi, Maria C. [1 ]
Massaccesi, Luca [3 ]
Basilico, Nicoletta [4 ]
Erba, Daniela [1 ]
机构
[1] Univ Milan, Dept Food Environm & Nutr Sci DeFENS, Human Nutr Unit, Via Celoria 2, I-20133 Milan, Italy
[2] Univ Milan, LITA, Dept Med Biotechnol & Translat Med, Via Flli Cervi 93, I-20090 Milan, Italy
[3] Univ Milan, Dept Biomed Surg & Dent Sci, Via Saldini 50, I-20133 Milan, Italy
[4] Univ Milan, Dept Biomed Sci Surg & Dent Sci, Via C Pascal 36, I-20133 Milan, Italy
来源
NUTRIENTS | 2018年 / 10卷 / 02期
关键词
ketone bodies; ketogenic diet; HMEC-1; cells; oxidative stress; Nrf2; pathway; TRANSCRIPTION FACTOR NRF2; OXIDATIVE STRESS; KETOGENIC DIET; HEME OXYGENASE-1; BETA-HYDROXYBUTYRATE; IN-VITRO; FECAL WATER; ACTIVATION; PATHWAY; ACETOACETATE;
D O I
10.3390/nu10020250
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The ketogenic diet (KD) is a high-fat, low-carbohydrate diet based on the induction of the synthesis of ketone bodies (KB). Despite its widespread use, the systemic impact of KD is not completely understood. The purpose of this study was to evaluate the effects of physiological levels of KB on HMEC-1 endothelial cells. To this aim, DNA oxidative damage and the activation of Nrf2, a known transcriptional factor involved in cell responses to oxidative stress, were assessed. The exposure of cells to KB exerted a moderate genotoxic effect, measured by a significant increase in DNA oxidative damage. However, cells pre-treated with KB for 48 h and subjected to a secondary oxidative insult (H2O2), significantly decreased DNA damage compared to control oxidized cells. This protection occurred by the activation of Nrf2 pathway. In KB-treated cells, we found increased levels of Nrf2 in nuclear extracts and higher gene expression of HO-1, a target gene of Nrf2, compared to control cells. These results suggest that KB, by inducing moderate oxidative stress, activate the transcription factor Nrf2, which induces the transcription of target genes involved in the cellular antioxidant defense system.
引用
收藏
页数:13
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