Decreased bacterial clearance from the lungs of mice following primary respiratory syncytial virus infection

被引:77
|
作者
Stark, JM
Stark, MA
Colasurdo, GN
LeVine, AM
机构
[1] Univ Texas, Hlth Sci Ctr, Dept Pediat, Houston, TX 77030 USA
[2] Childrens Hosp, Med Ctr, Div Pulm Biol, Cincinnati, OH 45229 USA
关键词
respiratory syncytial virus; Streptococcus pneumoniae; pneumonia; inflammation; bacterial superinfection; neutrophil;
D O I
10.1002/jmv.20631
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Virus respiratory infections often precede bacterial pneumonia in healthy individuals. In order to determine the potential role of respiratory syncytial virus (RSV) in bacterial secondary infections, a mouse sequential pulmonary infection model was developed. Mice were exposed to RSV then challenged with Streptococcus pneumoniae (StPn). Exposure of BALB/c mice to 10(6)-10(7) plaque forming units (pfu) of virus of RSV significantly decreased StPn clearance 1-7 days following RSV exposure, This finding was not restricted to StPn alone: exposure to RSV followed by Staphylococcus aureus (SA) or Pseudomonas aeruginosa(PA) resulted in similar decreases in bacterial clearance. Both bronchoalveolar lavage (BAL) cell counts and pulmonary histopathology demonstrated that RSV-StPn exposed mice had increased lung cellular inflammation compared to mice receiving StPn or RSV alone. The effect of RSV infection on bacterial clearance was dependent on the mouse genetic background: C57BL/6J mice (relatively resistant to RSV infection) demonstrated a modest change in StPn clearance following RSV exposure, whereas FVBN/J mice (similar to the BALB/cJ mice in RSV susceptibility) demonstrated a similar degree of RSV-associated decrease in StPn clearance 7 days following RSV exposure. Neutrophils from the RSV-StPn sequentially exposed BALB/cJ mice were functionally altered-produced greater levels of peroxide production but less myeloperoxidase (MPO) compared to mice receiving StPn alone. These data demonstrate that RSV infection decreases bacterial clearance, potentially predisposing to secondary bacterial pneumonia despite increased lung cellular inflammation, and suggest that functional changes occur in the recruited neutrophils that may contribute to the decreased bacterial clearance.
引用
收藏
页码:829 / 838
页数:10
相关论文
共 50 条
  • [31] DOES RESPIRATORY SYNCYTIAL VIRUS (RSV) PREDISPOSE TO BACTERIAL-INFECTION
    HALL, CB
    POWELL, KR
    GALA, CL
    PINCUS, PH
    CLINICAL RESEARCH, 1986, 34 (02): : A850 - A850
  • [32] DOES RESPIRATORY SYNCYTIAL VIRUS (RSV) PREDISPOSE TO BACTERIAL-INFECTION
    HALL, CB
    POWELL, KR
    GALA, CL
    PINCUS, PH
    PEDIATRIC RESEARCH, 1986, 20 (04) : A310 - A310
  • [33] Respiratory syncytial virus infection and the risk of serious bacterial infections - In reply
    Purcell, K
    Fergie, J
    ARCHIVES OF PEDIATRICS & ADOLESCENT MEDICINE, 2002, 156 (10): : 1056 - 1057
  • [34] Respiratory syncytial virus infection is strongly correlated with decreased mean platelet volume
    Renshaw, Andrew A.
    Drago, Barbara
    Toraya, Norma
    Gould, Edwin W.
    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2013, 17 (09) : E678 - E680
  • [35] Helminth induced monocytosis conveys protection from respiratory syncytial virus infection in mice
    Burgess, Matthew O.
    Janas, Piotr
    Berry, Karla
    Mayr, Hannah
    Mack, Matthias
    Jenkins, Stephen J.
    Bain, Calum C.
    Mcsorley, Henry J.
    Schwarze, Jurgen
    ALLERGY, 2024, 79 (08) : 2157 - 2172
  • [36] Overexpression of interleukin-4 delays virus clearance in mice infected with respiratory syncytial virus
    Fischer, JE
    Johnson, JE
    KuliZade, RK
    Johnson, TR
    Aung, S
    Parker, RA
    Graham, BS
    JOURNAL OF VIROLOGY, 1997, 71 (11) : 8672 - 8677
  • [37] In Vivo Evidence of Respiratory Syncytial Virus Persistence in a Subset of Pulmonary Dendritic Cells Following a Primary Infection
    Fonceca, Angela M.
    Lauzon-Joset, Jeff
    Scott, Naomi
    Stumbles, Philip A.
    Strickland, Deborah
    Everard, Mark L.
    VIRAL IMMUNOLOGY, 2023, 36 (07) : 466 - 474
  • [38] ROLE OF LYMPHOCYTE-T SUBSETS IN THE PATHOGENESIS OF PRIMARY INFECTION AND RECHALLENGE WITH RESPIRATORY SYNCYTIAL VIRUS IN MICE
    GRAHAM, BS
    BUNTON, LA
    WRIGHT, PF
    KARZON, DT
    JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (03): : 1026 - 1033
  • [39] Primary infection of mice with high titer inoculum respiratory syncytial virus:: characterization and response to antiviral therapy
    Bolger, G
    Lapeyre, N
    Dansereau, N
    Lagacé, L
    Berry, G
    Klosowski, K
    Mewhort, T
    Liuzzi, M
    CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2005, 83 (02) : 198 - 213
  • [40] CLEARANCE OF PERSISTENT RESPIRATORY SYNCYTIAL VIRUS-INFECTIONS IN IMMUNODEFICIENT MICE FOLLOWING TRANSFER OF PRIMED T-CELLS
    CANNON, MJ
    STOTT, EJ
    TAYLOR, G
    ASKONAS, BA
    IMMUNOLOGY, 1987, 62 (01) : 133 - 138