Effect of nutrient loading on bacterioplankton community composition in lake mesocosms

被引:190
作者
Haukka, K
Kolmonen, E
Hyder, R
Hietala, J
Vakkilainen, K
Kairesalo, T
Haari, H
Sivonen, K
机构
[1] Univ Helsinki, Viikki Bioctr, Dept Appl Chem & Microbiol, FIN-00014 Helsinki, Finland
[2] Univ Helsinki, Dept Ecol & Environm Sci, Lahti 15140, Finland
[3] Univ Helsinki, Dept Math, Helsinki 00014, Finland
关键词
D O I
10.1007/s00248-005-0049-7
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Changes in bacterioplankton community composition were followed in mesocosms set up in the littoral of Lake Vesijarvi, southern Finland, over two summers. Increasing nitrogen and phosphorus concentrations in the mesocosms represented different trophic states, from mesotrophic to hypertrophic. In 1998, the mesocosms were in a turbid state with a high biomass of phytoplankton, whereas in 1999, macrophytes proliferated and a clear-water state prevailed. The bacterial communities in the mesocosms also developed differently, as shown by denaturing gradient gel electrophoresis profiling of partial 16S rRNA gene fragments and by nonmetric multidimensional scaling analysis. In 1998, nutrient treatments affected the diversity and clustering of bacterial communities strongly, but in 1999, the bacterial communities were less diversified and not clearly affected by treatments. Canonical correspondence analysis indicated that bacterioplankton communities in the mesocosms were influenced by environmental physicochemical variables linked to the increasing level of eutrophication. Nitrogen concentration correlated directly with the bacterioplankton composition. In addition, the high nutrient levels had indirect effects through changes in the biomass and composition of phyto- and zooplankton. Sequencing analysis showed that the dominant bacterial divisions remained the same, but the dominant phylotypes changed during the 2-year period. The occurrence of Verrucomicrobia correlated with more eutrophic conditions, whereas the occurrence of Actinobacteria correlated with less eutrophic conditions.
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页码:137 / 146
页数:10
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